Microbiome Analysis of Skin and Airway Samples and Effect of Face Masks
- Conditions
- Healthy
- Interventions
- Other: Observational intervention
- Registration Number
- NCT04894422
- Lead Sponsor
- University Hospital, Antwerp
- Brief Summary
The goal of this study is to optimize two methods for microbiome analysis of skin and upper airway samples (16S amplicon sequencing and Shotgun sequencing). In addition, the researchers will investigate whether there is an effect of wearing a face mask on the microbial communities of the skin and upper airways. The volunteers will self-collect swabs of the skin and upper airways (nose and/or throat). Bacterial DNA and/or total RNA will be isolated and the microbial communities will be investigated with 16S amplicon sequencing and shotgun metagenomic sequencing.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 100
Inclusion Criteria
- Adult age 18 at the start of the study
Exclusion Criteria
- Pregnant women
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description No face mask Observational intervention Volunteers who did not wear a face mask Cotton face mask Observational intervention Voluntarily wearing a cotton face mask for 4 hours, following the government's guidelines during the COVID-19 pandemic. Surgical face mask Observational intervention Voluntarily wearing a surgical face mask for 4 hours, following the government's guidelines during the COVID-19 pandemic.
- Primary Outcome Measures
Name Time Method Optimalization of microbiome analysis of skin and upper airway samples Start 16S rRNA sequencing, shotgun metagenomic sequencing
Change from baseline in microbiome composition of the skin and upper airways after wearing a face mask Start, 4 hours 16S rRNA sequencing
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
University of Antwerp and University Hospital Antwerp
🇧🇪Antwerp, Belgium