Comprehensive DNA Methylation Profiling in Crohn's Disease
- Conditions
- Crohn DiseaseMethylationIllumina Human Methylation 850k BeadChip
- Interventions
- Genetic: Genome-wide DNA methylation
- Registration Number
- NCT03272152
- Lead Sponsor
- Jinling Hospital, China
- Brief Summary
Previous studies have indicated that abnormal DNA methylation frequently occurs in the mucosa in Crohn's disease. Comprehensive DNA methylation profiling of the inflamed and non-inflamed ileal mucosa of patients with Crohn's disease was performed. Genome-wide DNA methylation was determined using the Illumina HumanMethylation 850k BeadChip.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- Male
- Target Recruitment
- 14
Inclusion Criteria
- Active Crohn's disease(CDAI score≥ 150)
Exclusion Criteria
- No ileal lesions
- Under treatment of immunomodulators, biologic agents or steroids
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Inflamed CD group Genome-wide DNA methylation Inflamed ileal mucosa was obtained from inflammed lesions of active CD patients Non-inflamed CD group Genome-wide DNA methylation Non-inflamed ileal mucosa was obtained from normal sites of active CD patients Control group Genome-wide DNA methylation Control ileal mucosa was obtained from healthy patients
- Primary Outcome Measures
Name Time Method Intestinal inflammation under colonoscopy Jan 2017 to Sep 2017 Intestinal ulcer or enteritisis observed under colonoscopy
- Secondary Outcome Measures
Name Time Method CDAI Jan 2017 to Sep 2017 Crohns Disease Activity Index is measured.
Trial Locations
- Locations (1)
Jinling Hospital
🇨🇳Nanjing, Jiangsu, China