The effects of cardiopulmonary bypass on the function of white blood cells

Not Applicable

Completed

• Conditions: The role of lung inflation on neutrophil function in patients undergoing coronary artery bypass grafting; Circulatory System

• Registration Number: ISRCTN70523327
• Lead Sponsor: ewcastle Upon Tyne Hospitals NHS Foundation Trust (UK)

Brief Summary

2022 Results article in https://doi.org/10.1371/journal.pone.0271958 (added 28/10/2022)

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2015-11-05
• Study Completion: 2017-07-30
• Last Update Posted: 7 November 2022

Recruitment & Eligibility

• Status: Completed
• Sex: All
• Target Recruitment: 35

Inclusion Criteria

1. Aged 18 or over
2. Patients undergoing coronary artery bypass graft surgery

Exclusion Criteria

1. Patients under 18 years of age
2. Lack of informed, written consent
3. Emergency surgery precluding the capacity to give sufficient time for informed written consent
4. Redo surgery
5. Patients taking part in other interventional studies of CTIMPs that may significantly affect white blood cell function

Study & Design

• Study Type: Observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Differences in phagocytic capacity of neutrophils in blood is measured by way of direct microscopic visualisation of ingestion of Zymosan particles and by flow cytometric analysis using staph. aureus PHrodo bioparticles at baseline, during and after CPB.

Secondary Outcome Measures

Name	Time	Method
<br> 1. Differences in neutrophil priming status is measured by differences in cell surface expression markers (CD11b and CD62L) and release of reactive oxygen species by flow cytometric analysis at baseline, during and after CPB<br> 2. Differences in the serum cytokines of blood is measured using an ELISA assay at baseline, during and after CPB<br>