Genomic DNA in Human Blastocoele Fluid

• Conditions: Chromosomal Aneuploidies; Single Gene Desease
• Interventions: Other: blastocoele aspiration

• Registration Number: NCT01780415
• Lead Sponsor: Cervesi Hospital, Cattolica, Italy

Brief Summary

The objective will focus on the characterization of DNA fragments present in Blastocoele fluid, and the evaluation of these fragments as potential target for Preimplantation Genetic Diagnosis. To reach this goal, real-time PCR, Whole Genome Amplification techniques and -subsequently- Next Generation Sequencing and aCGH approaches will be used.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-01
• Study First Submitted: 2013-01-28
• Study First Submitted QC: 2013-01-30
• Study First Posted: 2013-01-31
• Status Verified: 2021-02
• Last Update Submitted: 2021-02-08
• Last Update Posted: 2021-02-09
• Primary Completion: 2021-12
• Study Completion: 2022-01

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Female
• Target Recruitment: 35

Inclusion Criteria

• IVF patient with supernumerary embryos to vitrify at blastocyst stage

Exclusion Criteria

• IVF patient without blastocyst formation

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Bastovit	blastocoele aspiration	all the patients that have supernumerary embryos to vitrify at blastocyst stage

Primary Outcome Measures

Name	Time	Method
Number of samples with DNA in blastocoele Fluid	6 month	the presence of DNA will be evaluated through rtPCR

Secondary Outcome Measures

Name	Time	Method
Number of euploid samples	8 months	Array CGH

Trial Locations

Locations (1)

Cervesi Hospital IVF Unit; 🇮🇹 Cattolica, Rimini, Italy