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Fludrocortisone in Healthy Volunteers (AFLUCO4)

Phase 2
Completed
Conditions
Healthy Volunteers
Interventions
Registration Number
NCT02140918
Lead Sponsor
Rennes University Hospital
Brief Summary

Fludrocortisone, in association with hydrocortisone, has demonstrated an improvement in survival in septic shock patients with relative adrenal insufficiency. However, the utility of low doses of steroids and in particular of mineralocorticoids in septic shock is still discussed.

The purpose of the investigators study is to investigate the effects of 3 increasing doses of fludrocortisone (100 μg, 200 μg, 400 μg) in order to determine which dose allows the best pressor response to phenylephrine in healthy volunteers, and simultaneously assess their respective hemodynamic and biological effects.

Detailed Description

In a previous study (AFLUCO2) in healthy volunteers with saline-induced hypoaldosteronism, the investigators found that single doses of both hydrocortisone and fludrocortisone induced a significant decrease in the pressor response to phenylephrine, probably due to a rapid non-genomic vasodilatory mechanism, and that these effects were additive.

The investigators also showed that, at the doses used in septic shock, hydrocortisone induced more pronounced mineralocorticoid effects than fludrocortisone and also induced systemic hemodynamic effects whereas fludrocortisone did not.

The investigators now want to perform a dose-response study under normal conditions (ie without saline-induced hypoaldosteronism) and after repeated administrations, to determine the optimal dose of fludrocortisone that allows an increase in the pressor response to phenylephrine and to characterize its concomitant hemodynamic and biological effects.

This placebo-controlled, randomized, double-blind, cross-over, 4-periods (fludrocortisone 100 μg/day, 200 μg/day, 400 μg/day, or placebo) study aims to investigate hemodynamic and biological effects of fludrocortisone administered orally during 5 days, in healthy volunteers.

Each period will be separated from the next one by a washout interval of at least 14 days.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
16
Inclusion Criteria
  • Men aged 20 to 25 years
  • Body Mass Index between 20 kg/m² and 25 kg/m²
  • Nonsmoker since at least 6 months
  • Normal clinical examination, electrocardiogram and transthoracic echocardiography
  • Normal routine biological parameters
  • Written informed consent
Exclusion Criteria
  • History of significant allergy
  • Resting heart rate < 50 bpm
  • Subjects with abnormal hepatic or renal function, or cardiovascular, pulmonary, endocrine or psychiatric disease
  • Ongoing medication during the study
  • Alcohol consumption more than 30g/day or drug addiction
  • Exclusion period mentioned on the national registry for clinical trials volunteers.
  • Subject under legal protection

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
PlaceboPlacebo* Placebo (four times daily) during 5 days * Investigations the sixth day
Fludrocortisone 200 μgFludrocortisone 200 μg* 200 μg/day (50 µg four times daily) of fludrocortisone during 5 days * Investigations the sixth day
Fludrocortisone 400 μgFludrocortisone 400 μg* 400 μg/day (100 µg four times daily) of fludrocortisone during 5 days * Investigations the sixth day
Fludrocortisone 100 μgFludrocortisone 100 μg* 100 μg/day (25 µg four times daily) of fludrocortisone during 5 days * Investigations the sixth day
Primary Outcome Measures
NameTimeMethod
Phenylephrine mean blood pressor dose-response relationship.1.5 hour after fludrocortisone administration
Secondary Outcome Measures
NameTimeMethod
Cardiac systolic and diastolic function assessed par transthoracic echocardiography during phenylephrine administrationbetween 1.5 and 2.5 hours after fludrocortisone administration
Central aortic hemodynamic parameters30min before and 1h, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration

Aortic systolic, diastolic and mean arterial pressures, central pulse pressure, central pressure augmentation index (AIx)

Systemic hemodynamic parameters30min before and 1h, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration

Systolic, diastolic and mean arterial pressures, heart rate, peripheral pulse pressure, cardiac output, stroke volume, systemic vascular resistances

Arterial stiffness : carotid-femoral pulse wave velocity30min before and 1h, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration
Plasma parameters30min before and 1h, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration

Blood electrolytes, urea, creatinine, glucose, renin, aldosterone

Urinary parameters30min before and 2h, 4h, 6h after fludrocortisone administration

Diuresis, urinary electrolytes, urea, creatinine, glucose

Area under the plasma concentration versus time curve (AUC)Just before and 5min, 10min, 20min, 30min, 1h, 1h30, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration
Plasma half-life of fludrocortisoneJust before and 5min, 10min, 20min, 30min, 1h, 1h30, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration
Total Body ClearanceJust before and 5min, 10min, 20min, 30min, 1h, 1h30, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration
Apparent volume of distributionJust before and 5min, 10min, 20min, 30min, 1h, 1h30, 2h, 3h, 4h, 5h, 6h after fludrocortisone administration

Trial Locations

Locations (1)

Rennes University Hospital

🇫🇷

Rennes, France

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