FiberKinetics study: The study of fiber fermentation, short chain fatty acid kinetics and utilization inside the gut and systemic circulatio
- Conditions
- microbiota compositie activiteit en microbiële productenmicrobial activityproducts of gut bacteria
- Registration Number
- NL-OMON48396
- Lead Sponsor
- Wageningen Universiteit
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 5
• Males
• Age 18-60yrs
• BMI between 18.5 and 30 kg/m2
• Regular bowel movement (defaecation on average once a day)
• Signed informed consent
• Having a history of medical or surgical events that, in the opinion of the
Investigator, may either put the subject at risk because of participation in
the study, or influence the results of the study (e.g. diabetes, cardiovascular
disease, gastrointestinal disease, renal failure, cancer, infectious disease,
nose/throat).
* Having a history of surgical events of the gastro-intestinal tract (e.g.
bariatric surgery/gastric bypass surgery).
• Presence of swallowing disorder
• Use of any prescribed or non-prescribed medication (other than paracetamol)
including antacids, analgesics, and herbal remedies during the three (3) weeks
prior to study start.
• Use of cholesterol lowering medication
• Carrying a pacemaker or any other (implanted) medical electronic device
• Smoker
• Unstable body weight (weight gain or loss >5kg in the past 3 months prior to
the study start)
• Use of antibiotics within 3 months of starting the study or planned during
the study
• Use of pro- or prebiotics (e.g. galacto-oligosaccharides,
fructo-oligosaccharides)
• Constipation/infrequent bowel movement
• Abuse of drugs/alcohol (alcohol: >4 consumptions/day or >21 consumptions/week)
• Participation in another biomedical study
• Having diarrhoea within 2 months prior to the study start
• Personnel of Wageningen University, Division of Human Nutrition, their
partner and their first and second degree relatives
• Current participation in other research from the Division of Human Nutrition
• Not willing to undergo fluoroscopy
• Having blood vessels that are too difficult for inserting a venflon
* Having a hemoglobin of <8.4 mmol/L
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Main study parameters/endpoints: (isotopic) enrichments of SCFAs (in the cecum<br /><br>and blood), and plasma organic acids, glucose, cholesterol, fatty acids</p><br>
- Secondary Outcome Measures
Name Time Method <p>Blood markers for microbial activity: Markers of specific microbial group<br /><br>activity that can be related to the host health status, like organic acids,<br /><br>hippurate, di- en tri-methylamine, acetaldehydes, glucuronic acid conjugates,<br /><br>and bile acid (conjugates).<br /><br><br /><br>Bacterial activity/microbiota composition in luminal samples.<br /><br><br /><br>Fibre degradation: To study acute fibre breakdown, the fibre degradation<br /><br>products, including mono-, di-, tri-, oligo- and polysaccharides.<br /><br><br /><br>Urine metabolic profiles: A fasting urine sample will be collected before at<br /><br>the start of the test day and at the end of the test day. Urine samples will be<br /><br>analysed to analyse bile acids, organic acids, aminoa cids, including bacterial<br /><br>products and their taurine, glycine, and glucuronic acid conjugates.</p><br>