FiberKinetics2 study: A feasibility study on fiber fermentation, and short chain fatty acid kinetics and utilization inside the gut and systemic circulatio

Completed

• Conditions: de darm: microbiota compositie activiteit en microbiële producten; microbial activity; products of gut bacteria

• Registration Number: NL-OMON49956
• Lead Sponsor: Wageningen Universiteit

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 15 April 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 10

Inclusion Criteria

* Males
* Age 18-60yrs
* BMI between 18.5 and 30 kg/m2
* Regular bowel movement (defaecation on average once a day)
* Signed informed consent

Exclusion Criteria

* Having a history of medical or surgical events that, in the opinion of the
Investigator, may either put the subject at risk because of participation in
the study, or influence the results of the study (e.g. diabetes, cardiovascular
disease, gastrointestinal disease, renal failure, cancer, infectious disease,
nose/throat).
* Having a history of surgical events of the gastro-intestinal tract (e.g.
bariatric surgery/gastric bypass surgery)
* Presence of swallowing disorder
* Use of any prescribed or non-prescribed medication (other than paracetamol)
including antacids, analgesics, and herbal remedies during the three (3) weeks
prior to study start.
* Being lactose intolerant
* Follows a vegan diet
* Use of antibiotics within 3 months of starting the study or planned during
the study
* Use of pro- or prebiotics (e.g. galacto-oligosaccharides,
fructo-oligosaccharides)
* Constipation/infrequent bowel movement (less than 3 times defaecation per
week)
* Abuse of drugs/alcohol (alcohol: >4 consumptions/day or >21 consumptions/week)
* Smoker
* Having diarrhoea within 1 month prior to the study start
* Personnel of Wageningen University, Division of Human Nutrition, their
partner and their first and second degree relatives
* Participation in another biomedical study or other research from the Division
of Human Nutrition
* Not willing to be exposed to fluoroscopy
* Is sensitive for iodinated components
* Having a hemoglobin of <8.5 mmol/L
* Having blood vessels that are too difficult for inserting a cannula
* Not having a general practitioner

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>- To show placement of a naso-intestinal catheter in the proximal colon, and<br /><br>sampling in this intestinal region.<br /><br>- (The effect of NDC on) intestine luminal NDC and its degradation products<br /><br>SCFA. Also (13C label incorporation in) plasma metabolic markers such as<br /><br>organic acids, amino acids, glucose, cholesterol, fatty acids and bile acids.<br /><br>- To investigate if intake of dietary fiber for a couple of days will effect<br /><br>in vivo acute fiber fermentation.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Intestinal lumen microbiota and activity after NDC bolus.</p><br>