Evaluation of antimicrobial efficacy of Etidronic acid against E. faecalis in primary teeth: An in vivo study

An interventional, double blinded, randomised parallel group trial will be conducted in 60 teeth from the patients requiring pulpectomy of 3 to 8 years old after obtaining a written informed consent from the parents of child. 60 teeth requiring pulpectomy will be divided into two groups using following irrigants: Group I (n=30 teeth): 2% Chlorhexidine gluconate and Group II (n=30 teeth): 9% Etidronic acid. Appropriate local anesthesia will be given and the tooth will be isolated with rubber dam. The gross carious tissue will be removed and initial access opening will be done with No. 3 round carbide bur. Working length will be determined with preoperative intraoral periapical radiograph. First microbiological sample will be obtained with 2 sterile absorbent paper points each for 30 seconds from the root canal (pre irrigation) and will be immediately immersed into test tube with Brain Heart Infusion Broth (BHI). Conventional biomechanical preparation will be done upto 35 k-file. During instrumentation after each file, Group I: 0.5 ml of 2% Chlorhexidine gluconate for 5 seconds will be used as an irrigant and Group II: 0.5 ml of 9% Etidronic acid for 5 seconds will be used as an irrigant. Final irrigation of canals will be done at the end of instrumentation and dried before obturating and second microbiological sample will be collected with another 2 sterile absorbent paper points each for 30 seconds. The root canal will be filled with a obturating material and a postoperative intraoral periapical radiograph will be taken. The collected samples will be subjected to microbiological analysis to determine the number of colony forming units against E. faecalis. The obtained results will be subjected to statistical analysis.