Involvement of Polymorphonuclear Neutrophils and Platelets in Severe Form of COVID-19
Trial Snapshot
- Phase
- Not Applicable
- Enrollment
- 200
- Locations
- 1
- Primary Endpoint
- Percentage of circulating neutrophils in reverse migration
Study Overview
Brief Summary
The main objective is to quantify the population of reverse migrating neutrophils in patients with COVID-19.
Detailed Description
This is a non-interventional transversal research whose main objective is to quantify the population of reverse migrating neutrophils in patients with COVID-19.
Design:
A multicentric (3 sites) clinical trial
Sample size :
200 patients
Study Design
- Study Type
- Observational
- Observational Model
- Cohort
- Time Perspective
- Prospective
Eligibility Criteria
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Patients admitted to the intensive care units of Saint-Antoine, Tenon and Pitié-Salpêtrière hospitals (Paris, France)
- •With moderate to severe respiratory distress syndrome and SARS-CoV-2 infection confirmed by RT-PCR from a nasopharyngeal swab
- •informed and not opposed to participation in research
- •included within 4 hours following the paatient's admission
Exclusion Criteria
- •Patients under guardianship / curatorship
- •Patients under AME
Outcomes
Primary Outcomes
Percentage of circulating neutrophils in reverse migration
Time Frame: Within 4 hours following the patient admission in Intensive Care Units (ICU).
Percentage of circulating neutrophils in reverse migration determined on a sample taken during the admission of COVID 19 patients to Intensive Care Units by flow cytometry: the samples will be incubated for 45 minutes with anti-human CXCR1, CD11c, CD11b, and CD62L antibodies. Erythrocytes will be lysed and fixed leukocytes will be analyzed by flow cytometry.
Secondary Outcomes
- Analysis of neutrophils and platelets(Within 4 hours following the patient admission in Intensive Care Units (ICU).)
- SAPS II score(From admission to the follow-up visits (Day 3, Day 7 and as part of usual care every 7 days until the patient is discharged and within 2 months top).)
- SOFA score(From admission to the follow-up visits (Day 3, Day 7 and as part of usual care every 7 days until the patient is discharged and within 2 months top).)
- Mortality rate(2 months)