Time-course of Mitochondrial Biogenic Gene and Protein Expression in Exercised Human Skeletal Muscle
- Conditions
- Mitochondrial Biogenesis
- Interventions
- Other: LOOther: HI
- Registration Number
- NCT03975777
- Lead Sponsor
- Brendon Gurd, PhD
- Brief Summary
This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- Male
- Target Recruitment
- 10
- recreationally active (no more than three hours of structured weekly physical activity)
- not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
- body mass index < 30 kg/m2
- presence of cardiometabolic disease
- taking regular prescription medication for the treatment of cardiometabolic disease
- current cigarette smoker
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description high-intensity exercise - low-intensity exercise HI high-intensity exercise session followed by a low-intensity exercise session separated by a minimum of 14 days low-intensity exercise - high-intensity exercise LO low-intensity exercise session followed by a high-intensity exercise session separated by a minimum of 14 days low-intensity exercise - high-intensity exercise HI low-intensity exercise session followed by a high-intensity exercise session separated by a minimum of 14 days high-intensity exercise - low-intensity exercise LO high-intensity exercise session followed by a low-intensity exercise session separated by a minimum of 14 days
- Primary Outcome Measures
Name Time Method Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise PGC-1α gene expression (indirect indicator of PGC-1α activity). Transcriptional co-activator involved in the control of mitochondrial biogenesis.
- Secondary Outcome Measures
Name Time Method Change in PGC-1α protein content before exercise to 1, 3, 6, 9, and 12 hours post-exercise PGC-1α protein content
Change in Nrf2 protein content before exercise to 1, 3, 6, 9, and 12 hours post-exercise Nrf2 protein content
Change in LRP130 protein content before exercise and 1, 3, 6, 9, and 12 hours post-exercise LRP130 protein content
Change in COXI mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise COXI gene expression. Mitochondrial encoded subunit of the electron transport chain complex IV (COX)
Change in NRF-2 mRNA expression before exercise and 1, 3, 6, 9, and 12 hours post-exercise NRF-2 gene expression. Transcription factor involved in the control of mitochondrial biogenesis.
Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise Nrf2 gene expression (indirect indicator of Nrf2 activity). Transcription factor involved in the control of mitochondrial biogenesis.
Change in NRF-1 protein content before exercise to 1, 3, 6, 9, and 12 hours post-exercise NRF-1 protein content
Change in ND4 mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise ND4 gene expression. Mitochondrial encoded subunit of the electron transport chain complex 1 (NADH dehydrogenase).
Change in mitochondrial transcription factor A (TFAM) mRNA expression before exercise and 1, 3, 6, 9, and 12 hours post-exercise TFAM gene expression. Transcription factor controlling the expression of mitochondrial-encoded genes.
Change in TFAM protein content before exercise and 1, 3, 6, 9, and 12 hours post-exercise TFAM protein content
Change in PPAR-beta mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise PPAR-beta gene expression (indirect indicator of PPAR-beta activity). Transcription factor involved in the control of mitochondrial biogenesis.
Change in estrogen related receptor (ERR)-gamma mRNA expression before exercise and 1, 3, 6, 9, and 12 hours post-exercise ERR-gamma gene expression. Transcription factor involved in the control of mitochondrial biogenesis.
Change in leucine-rich pentatricopeptide repeat containing protein 130 (LRP130) mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise LRP130 gene expression. Transcriptional co-activator and RNA-binding protein involved in the transcriptional control and stabilization of mitochondrial mRNAs
Change in p53 mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise p53 gene expression. Transcription factor involved in the control of mitochondrial biogenesis.
Change in cytochrome c oxidase subunit 4 (COXIV) mRNA expression. before exercise to 1, 3, 6, 9, and 12 hours post-exercise COXIV gene expression. Nuclear encoded subunit of the electron transport chain complex 4 (COX)
Change in COXII mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise COXII gene expression.Mitochondrial encoded subunit of the electron transport chain complex IV (COX).
Change in nicotinamide adenine dinucleotide (NADH) subunit 1 (ND1) mRNA expression before exercise and 1, 3, 6, 9, and 12 hours post-exercise ND1 gene expression. Mitochondrial encoded subunit of the electron transport chain complex 1 (NADH dehydrogenase)
Change in citrate synthase (CS) mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise CS gene expression. The first rate limiting enzyme of the Kreb's cycle.
Change in nuclear respiratory factor-1 (NRF-1) mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise NRF-1 gene expression. Transcription factor involved in the control of mitochondrial biogenesis.
Change in mitochondrial transcription factor B1 (TFB1M) mRNA expression before exercise to 1, 3, 6, 9, and 12 hours post-exercise TFB1M gene expression. Transcription factor controlling the expression of mitochondrial-encoded genes.
Change in mitochondrial transcription factor B2 (TFB2M) mRNA expression before exercise and 1, 3, 6, 9, and 12 hours post-exercise TFB2M gene expression. Transcription factor controlling the expression of mitochondrial-encoded genes.
Trial Locations
- Locations (1)
School of Kinesiology and Health Studies
🇨🇦Kingston, Ontario, Canada