Impact of Exercise Intensity on the Time-course of Mitochondrial Biogenic Gene and Protein Expression in Human Skeletal Muscle.

Brendon Gurd, PhD1 site in 1 country10 target enrollmentJune 1, 2019

ConditionsMitochondrial Biogenesis

Overview

Phase: Not Applicable
Intervention: Not specified
Conditions: Mitochondrial Biogenesis
Sponsor: Brendon Gurd, PhD
Enrollment: 10
Locations: 1
Primary Endpoint: Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
Last Updated: 6 years ago

Overview Investigators Eligibility Criteria Outcomes Sites Similar Trials

Overview

Brief Summary

This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.

Registry

clinicaltrials.gov

Start Date

June 1, 2019

End Date

April 30, 2020

Last Updated

6 years ago

Study Type

Interventional

Study Design

Crossover

Sex

Male

Investigators

Sponsor

Brendon Gurd, PhD

Responsible Party

Sponsor Investigator

Principal Investigator

Brendon Gurd, PhD

Associate Professor

Queen's University

Eligibility Criteria

Inclusion Criteria

•recreationally active (no more than three hours of structured weekly physical activity)
•not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
•body mass index \< 30 kg/m2

Exclusion Criteria

•presence of cardiometabolic disease
•taking regular prescription medication for the treatment of cardiometabolic disease
•current cigarette smoker

Outcomes

Primary Outcomes

Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression

Time Frame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise

PGC-1α gene expression (indirect indicator of PGC-1α activity). Transcriptional co-activator involved in the control of mitochondrial biogenesis.

Secondary Outcomes

Change in PGC-1α protein content(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in Nrf2 protein content(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in LRP130 protein content(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
Change in COXI mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in NRF-2 mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in NRF-1 protein content(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in ND4 mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in PPAR-beta mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in estrogen related receptor (ERR)-gamma mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
Change in leucine-rich pentatricopeptide repeat containing protein 130 (LRP130) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in mitochondrial transcription factor A (TFAM) mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
Change in TFAM protein content(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
Change in p53 mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in cytochrome c oxidase subunit 4 (COXIV) mRNA expression.(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in COXII mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in nicotinamide adenine dinucleotide (NADH) subunit 1 (ND1) mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
Change in citrate synthase (CS) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in nuclear respiratory factor-1 (NRF-1) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in mitochondrial transcription factor B1 (TFB1M) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
Change in mitochondrial transcription factor B2 (TFB2M) mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)