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Optimal Planning of a Day 3 Cryopreserved(Frozen)-Thawed Embryo Transfer in a Natural Cycle With hCG Administration or After Spontaneous LH Peak?

Phase 4
Completed
Conditions
Supervision of Pregnancy Resulting From In-vitro Fertilization
Interventions
Other: LH peak
Registration Number
NCT02145819
Lead Sponsor
Universitair Ziekenhuis Brussel
Brief Summary

The aim of the study is to determine whether spontaneous LH peak is superior to human chorionic gonadotropin before a transfer of a day 3 frozen embryo.

Detailed Description

For surplus embryos after fresh IVF-cycles, cryopreservation has become common medical practice. These frozen embryos are mostly replaced in an artificial cycle with exogenous estrogen and progesterone or in a natural cycle. Often, hCG is administered as an ovulation induction agent for scheduling purposes.

Successful implantation requires a co-ordinated series of events allowing a timely dialogue between a receptive endometrium and the intrusive blastocyst . The period of receptivity is thought to be 3 days in human. It is suggested that blastocyst apposition begins about day LH+6 and is completed by day LH+10

In general, the aim is to transfer the embryo during the 'window of implantation', what is defined as the period during which the uterus is receptive for implantation of the free-lying blastocyst. This has been a subject of debate since many years.

A prospective study by Fatemi et al. (2010) revealed a significantly higher ongoing pregnancy rate after transferring frozen-thawed embryos in natural cycles with a spontaneous LH peak compared with natural cycles controlled by hCG for final oocyte maturation and ovulation (31.1% vs. 14.3%, respectively). In this trial, FrET (frozen embryo transfer) was planned 5 days after the LH surge or 5 days after the administration of 5000IU of hCG.

In order to optimize the synchronization in the hCG group, and therefore enhance the pregnancy rates, the aim is to plan a FrET 6 days after hCG administration instead of 5 days. The rationale behind is that day 3 frozen embryos are thawed the day before embryo transfer, which means they are already at day 4 of the embryonic development.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
240
Inclusion Criteria
  • Natural cycles, in which a frozen-thawed day 3 embryo is replaced.
  • Signed informed consent.
  • Regular cycle (i.e. between 26 and 35 days)
  • Normal transvaginal ultrasound at screening, without evidence of clinically significant abnormality consistent with finding adequate for ART with respect to uterus and adnexa.
  • Embryos frozen by vitrification.
  • Single or dual embryo transfer.
Exclusion Criteria
  • Known allergic reactions to progesterone products.
  • Intake of experimental drug within 30 days prior to study start.
  • Contraindication for pregnancy.
  • Embryos of women above 39 years of age at the time of embryo freezing.
  • Recipients of oocyte donation cycles

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
A: spontaneous LH peakLH peakIn group A, embryos are thawed 4 days after LH surge, with a re-evaluation and transfer 5 days after LH surge.
B: hCGhCGIn group B, embryos are thawed 5 days after hCG administration, with a re-evaluation and transfer 6 days after hCG.
Primary Outcome Measures
NameTimeMethod
clinical pregnancy rate7 weeks

We evaluate the pregnancy, at 7 weeks amenorrhoea. Outcome is clinical pregnancy rate

Secondary Outcome Measures
NameTimeMethod
number of monitoring visits at the clinic per cycle7 weeks

Trial Locations

Locations (1)

Centre of Reproductive Medicine CRG

🇧🇪

Jette, Brussels, Belgium

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