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Denileukin Diftitox Followed by Vaccine Therapy in Treating Patients With Metastatic Cancer

Phase 1
Completed
Conditions
Breast Cancer
Colorectal Cancer
Lung Cancer
Pancreatic Cancer
Unspecified Adult Solid Tumor, Protocol Specific
Interventions
Biological: denileukin diftitox
Biological: recombinant fowlpox-CEA(6D)/TRICOM vaccine
Biological: therapeutic autologous dendritic cells
Registration Number
NCT00128622
Lead Sponsor
H. Kim Lyerly
Brief Summary

RATIONALE: Combinations of biological substances in denileukin diftitox may be able to carry cancer-killing substances directly to the cancer cells. Vaccines made from a gene-modified virus and a person's white blood cells may help the body build an effective immune response to kill cancer cells. Giving denileukin diftitox together with vaccine therapy may kill more cancer cells.

PURPOSE: This phase I trial is studying the side effects of giving denileukin diftitox together with vaccine therapy in treating patients with metastatic cancer that expresses carcinoembryonic antigen.

Detailed Description

OBJECTIVES:

Primary

* Determine the safety and feasibility of two different schedules of denileukin diftitox followed by active immunotherapy comprising autologous dendritic cells infected with recombinant fowlpox-CEA(6D)-TRICOM vaccine in patients with metastatic CEA-expressing malignancies.

Secondary

* Determine the immune response to this regimen in these patients.

* Determine, preliminarily, clinical response rate and/or time to progression in patients with assessable disease treated with this regimen.

OUTLINE: Patients undergo leukapheresis for collection of peripheral blood mononuclear cells (PBMCs). PBMCs are cultured with sargramostim (GM-CSF) and interleukin-4 for the production of dendritic cells( DC). DC are mixed with recombinant fowlpox-TRICOM to produce the vaccine. Patients are assigned to 1 of 2 cohorts according to timing of study enrollment.

* Cohort 1: Patients receive denileukin diftitox IV over at least 15 minutes once in week 0 and vaccine therapy comprising autologous DC infected with recombinant fowlpox-CEA (6D)-TRICOM vaccine intradermally and subcutaneously once in weeks 0 (beginning 4 days after the denileukin diftitox infusion), 3, 6, and 9. If \< 2 of 6 patients experience dose-limiting toxicity, a second cohort of patients is enrolled.

* Cohort 2: Patients receive denileukin diftitox as in cohort 1 once in weeks 0, 3, 6, and 9 and vaccine as in cohort 1.

In both cohorts, treatment continues in the absence of disease progression or unacceptable toxicity.

After completion of study treatment, patients are followed annually for up to 15 years.

PROJECTED ACCRUAL: A total of 6-12 patients (6 per cohort) will be accrued for this study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
24
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Denileukin Diftitox plus vaccinedenileukin diftitoxThis is a single arm Phase I safety study.
Denileukin Diftitox plus vaccinerecombinant fowlpox-CEA(6D)/TRICOM vaccineThis is a single arm Phase I safety study.
Denileukin Diftitox plus vaccinetherapeutic autologous dendritic cellsThis is a single arm Phase I safety study.
Primary Outcome Measures
NameTimeMethod
Safety as measured by rate of adverse events during study drug treatment3 months
Secondary Outcome Measures
NameTimeMethod
Rate of immune response as measured by ELISPot at week 103 months

Trial Locations

Locations (2)

Lombardi Comprehensive Cancer Center at Georgetown University Medical Center

🇺🇸

Washington, District of Columbia, United States

Duke Comprehensive Cancer Center

🇺🇸

Durham, North Carolina, United States

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