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Compare Actions in Healthy Volunteer of 50 mg Etanercept Injection Using an Auto-injector Device and Manual Injection

Phase 1
Completed
Conditions
Healthy Men and Women
Interventions
Device: Auto-injector device
Other: Etanercept (ENBREL®) via Manual injection
Registration Number
NCT02799498
Lead Sponsor
Amgen
Brief Summary

The purpose of the study is to compare Pharmacokinetics of liquid etanercept that is administered to healthy subjects aged 18-55 by an auto-injector device and manual injection (each subject received both injections).

Detailed Description

This single-center, randomized, open-label, 2-period, 2-sequence, 2-treatment, crossover study in healthy men and women compared the pharmacokinetics (PK) and safety profiles of two 50-mg subcutaneous (SC) injections of etanercept liquid (in a 1.0-mL prefilled syringe): (1) using a disposable auto-injector device, and (2) using a standard manual injection. Each subject received both injections in the abdomen, separated by a washout period of 28 days.

Recruitment & Eligibility

Status
COMPLETED
Sex
Not specified
Target Recruitment
36
Inclusion Criteria
  • Healty men and women
  • Aged 18-55 years at time of screening
  • BMI 18-31 kg/m2 inclusive
  • Free of any clinically significant disease
  • Willing to reside in research facility 4 consecutive nights 2 times and to attend follow up visits
  • Willing to sign consent
  • Negative HIV, hepatitis B and C, and urine pregnancy tests
Exclusion Criteria
  • Unstable medical condition (hospitalized within 30 days, myocardial infarction or major surgery within 6 months, or seizure within 12 months of study day 1)
  • Current active infecton, history of infections, or condition which may predispose infection (such as diabetes)
  • Clinically significant abnormality in laboratory samples done while screening
  • history of tuberculosis
  • donated blood within 30 days of screening
  • Use of prescription or over-the-counter medication during the study/
  • History of smoking or use of tobacco within 30 days of screening
  • Positive urine scree for alcohol or drugs of abuse at screening or the day prior to dosing
  • Unwilling to pracitce contraception for the duration ot the study
  • Any other condition which could interfere with obtaining data required by the protocol

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
A-etanercept (ENBREL®) by auto-injectorEtanercept (ENBREL®)Single dose of etanercept (ENBREL®) in a pre-filled syringe administered with an auto-injector device manufactured by Scandinavian Health Limited (SHL)
A-etanercept (ENBREL®) by auto-injectorAuto-injector deviceSingle dose of etanercept (ENBREL®) in a pre-filled syringe administered with an auto-injector device manufactured by Scandinavian Health Limited (SHL)
B-etanercept (ENBREL®) by Manual injectionEtanercept (ENBREL®) via Manual injectionSingle dose of etanercept (ENBREL®) in a syringe given by manual injection (reference treatment)
Primary Outcome Measures
NameTimeMethod
Ratio of the geometric means of etanercept by auto-injector to etanercept by manual injection for the PK parameter of AUC (0-t)28 days

28 days after receiving treatment in Period 1, subjects return to the facility on an outpatient basis to receive the alternate treatment in Period 2. Procedures performed in the first period are repeated in the second period.

Secondary Outcome Measures
NameTimeMethod
Measure of vital signs changes from baseline to end of each treatment periodBaseline and 28 days following each treatment

Changes from baseline in vital signs (includes blood pressure-systolic and diastolic; and heart rate per minute) and physical examinations

PK parameters of the area under the serum drug concentration (t z)28 days: timepoint at which outcome measure is assessed following each treatment arm

terminal phase half-life

Blood samples obtained to measure seroreactivitiy to etanercept at baseline and following treatmentPredose in each treatment period and 28 days following dosing in treatment period B

Blood samples collected to measure seroreactivity to etanercept

Safety Events measured by adverse events and how they relate to study drug28 days-timepoint at which outcome measure is assessed following each treatment arm

Incidence, severity, and relationship to study drug of adverse events for each subject

Profile PK parameters of AUC (0-∞)28 days: timepoint at which outcome measure is assessed following each treatment arm

PK parameters of the area under the serum drug concentration-time curve from time zero to infinity

Any Clinically Significant changes in clinical laboratory tests will be notedCollected at screening, Day -1, Day 4 after dosing each treatment period, and on day 15 after dosing in Period 2

Laboratory variables summarized separately from adverse events and compared to normal ranges; samples will be analyzed by standard procedures

Profile PK parameters of AUC (C max )28 days: timepoint at which outcome measure is assessed following each treatment arm

maximum observed concentration

PK parameters of the area under the serum drug concentration (t 1/2)28 days: timepoint at which outcome measure is assessed following each treatment arm

terminal phase half-life

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