Regulatory B Cells and Chronic Immune Thrombocytopenia

Completed

• Conditions: Regulatory B Cells; Purpura, Thrombocytopenic, Idiopathic
• Interventions: Biological: Biological tests

• Registration Number: NCT02891109
• Lead Sponsor: University Hospital, Brest

Brief Summary

The chronic immune thrombopenia is an autoimmune disease caused by B cells. These cells produce anti platelets and megakaryocytes antibodies. Some B cells, named regulatory B cells, are known to control other cells. Their action in chronic immune thrombopenia is actually unknown.

Detailed Description

The chronic immune thrombopenia is an autoimmune disease caused by B cells. These cells produce anti platelets and megakaryocytes antibodies. Some B cells, named regulatory B cells, are known to control other cells. Their action in chronic immune thrombopenia is actually unknown. In our cohort of chronic immune thrombopenia and controls, we realized a flow cytometry analyze of B and T cells and a co-culture to determine regulatory B cells functionality.

Study Timeline

• Study Start: 2015-01
• Primary Completion: 2015-09
• Study Completion: 2015-09
• Status Verified: 2016-08
• Study First Submitted: 2016-09-01
• Study First Submitted QC: 2016-09-01
• Last Update Submitted: 2016-09-01
• Study First Posted: 2016-09-07
• Last Update Posted: 2016-09-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 36

Inclusion Criteria

• Purpura, Thrombocytopenic, Idiopathic, chronic phase (>12 months)
• no treatment or thrombopoietin receptor agonists

Exclusion Criteria

• < 18 years
• Purpura, Thrombocytopenic, Idiopathic, acute phase
• pregnancy
• others causes of thrombopenia

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
patients group	Biological tests	Adults patients with chronic immune thrombocytopenia
control group	Biological tests	Adults without immune thrombocytopenia

Primary Outcome Measures

Name	Time	Method
Regulatory B cells functionality	1 week	inhibition of T cells proliferation with co-culture B and T cells

Secondary Outcome Measures

Name	Time	Method
Cytometry immunophenotyping of B and T cells	1 week	characterization of B and T cells by flow cytometry

Trial Locations

Locations (1)

CHRU de Brest; 🇫🇷 Brest, France