An Observational Study on the Relationship Between Different Exercise Intensities and Energy Expenditure Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Differences in total energy expenditure (TEE) measured by the doubly labeled water (DLW) method across experimental groups
研究概览
简要总结
The goal of this observational study is to learn about the effects of physical activity in adult on metabolic response. The main question it aims to answer is:
- if physical activity is unlimited to increase the total energy expenditure (TEE)?
Participants will engage in the following measurements:
- Record their daily physical activity by GT3X.
- Measure TEE by doubly-labeled water (DLW) method
- Measure basal energy expenditure(BEE) and thermic effect of food(TEF) by respiratory chamber
详细描述
The purpose of this study is to explore the effect of various physical activities on metabolic and behavior response. Energy expenditure, body composition, and metabolism will be measured by the following measurements:
- TEE:
TEE will be measured using the DLW method. Urine samples from all participants in the DLW subset will be stored at -20℃ and shipped on dry ice for analysis in the laboratory of Prof. John Speakman at the Shenzhen Institutes of Advanced Technology, Chinese academy of sciences. Isotopes will be measured in benchtop near-infrared isotope gas analyzer, and mean CO2 production will be calculated from isotope ratios using the recently derived equation. TEE will then be calculated using mean CO2 production.
- REE and TEF:
REE and TEF will be measured by indirect calorimetry while participants are resting supine in a respiratory chamber with thermoneutral conditions. Oxygen consumption and CO2 production will be measured to calculate energy expenditure. REE will be consistently measured throughout sleeping at night and in the morning after overnight fasting, and TEF will be measured after breakfast.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •BMI ≥ 18.5 kg/m2
排除标准
- •Individuals who have undergone major surgery in the past 6 months (excluding tooth extraction);
- •Individuals with severe metabolic and cardiovascular diseases such as diabetes, hypoglycemia, and hypertension;
- •Individuals with exercise-related injuries, including joint diseases and fractures;
- •Individuals diagnosed with infectious diseases such as HIV, tuberculosis, malaria, etc.;
- •Individuals with mental and neurological disorders including anorexia nervosa;
- •Individuals who have attempted weight loss or gain through various methods in the past three months;
- •Individuals in preconception, pregnancy, and lactation periods;
- •Individuals with autism and those with metal implants in their bodies.
结局指标
主要结局
Differences in total energy expenditure (TEE) measured by the doubly labeled water (DLW) method across experimental groups
时间窗: 14days
- The change in TEE will be evaluated using the DLW. Urine samples from all participants in the DLW subset will be stored at -20℃ and shipped on dry ice for analysis in the laboratory of Dr. John Speakman at the University of Aberdeen, or the Shenzhen Institutes of Advanced Technology, Chinese academy of sciences. Isotopes will be measured in benchtop near-infrared isotope gas analyzer, and mean CO2 production will be calculated from isotope ratios using the recently derived equation (Speakman et al 2021: Cell reports medicine). TEE will then be calculated using mean CO2 production using the Weir equation.
Differences in non-exercise physical activity across experimental groups
时间窗: 14days
- Physical activity of the participants will be recorded using GT3X accelerometer worn near the hip for a consecutive period of 14 days.
Differences in resting energy expenditure (REE) measured by respiratory chamber across experimental groups
时间窗: 1 day
- REE will be evaluated by respiratory chamber.
Differences in thermic effect of food (TEF) measured by respiratory chamber across experimental groups
时间窗: 1 day
- TEF will be evaluated by respiratory chamber.
Differences in exercise energy expenditure (EEE) measured by COSMED across experimental groups
时间窗: 1 hour
- EEE will be evaluated using an indirect calorimetry COSMED.
Differences in food intake across experimental groups
时间窗: 2 days
Photographic and food weighing methods to record food intake for two random days throughout the period of DLW measurement
次要结局
- Differences in sedentary time measured by GT3X accelerometer across experimental groups(14 days)
- Differences in glucose across experimental groups(7 days)
- Differences in fat mass across experimental groups(10 minutes)
- Differences in fat-free mass across experimental groups(2 minutes)
- Differences in bone mass intensity across experimental groups(7 minutes)
- Differences in circulating hormones across experimental groups(1 minutes for collection, 7 days for analysis.)
- Differences in intestinal microbiota across experimental groups(5 minutes for collection, 7 days for analysis.)
研究者
John R. Speakman
Professor
Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences