The Effect of Coffee on Energy Expenditure and Caffeine Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Total energy expenditure
研究概览
简要总结
Obesity is a risk factor for chronic diseases, and improving energy expenditure is a way to prevent and treat obesity. The purpose of this project is to explore the relationship between the effect of coffee on energy expenditure and caffeine metabolism, which has important significance in the rational application of caffeine.
详细描述
The investigators will study the relationship between the effect of coffee on energy expenditure and caffeine metabolism. The investigators will recruit healthy participants. Participants will be forbidden to consume stimulant drinks such as coffee for 24 hours before the experiment. When the participants arrive at the lab on the day of the experiment, they will sign an informed consent form, a health history questionnaire and a daily coffee consumption questionnaire. The changes of drinking water/coffee on total energy expenditure (TEE), body temperature, heart rate, blood pressure, electrocardiogram, memory，cognition, sleep time and so on will be observed. The concentration of caffeine and its metabolites in the blood and urine will be measured by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy adults.
- •Must be able to drink coffee.
排除标准
- •Diabetes.
- •Hypoglycemia.
- •Osteoporosis.
- •Gastric ulcer.
- •Pancreatitis.
- •Intestinal obstruction.
- •Blood phobia.
- •Pathological hypo or hyper tension.
- •Impaired glucose tolerance.
- •Claustrophobia.
结局指标
主要结局
Total energy expenditure
时间窗: 2 days
Total energy expenditure will be measured by Chamber (OMNICAL).
The concentration of caffeine and its metabolites in urine
时间窗: 2 days
The contents of caffeine and it's metabolites will be measured by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS ).
The concentration of caffeine and its metabolites in serum
时间窗: 1 day
The concentration of caffeine and its metabolites in serum will be measured by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS ).
次要结局
- Body temperature(2 days)
- Electrocardiogram (ECG)(2 days)
- Blood pressure(2 days)
- Height(The morning of the first day of the experiment, 5 mins)
- Body weight(The morning of the first day of the experiment, 5 mins)
- Fat mass(The morning of the first day of the experiment, 10 mins)
- Fat free mass(on the morning of the experiment, 5 mins)
- Heart rate(2 days)
- Hunger rating(2 days)
- Memory and cognitive tests(The morning of the experiment, 60 mins)
- Physical activity(7 days)
- Sleep duration(2 days)
- Waist circumferences(The morning of the first day of the experiment,10 mins.)
研究者
John R. Speakman
Professor
Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences