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Influence of Probiotic and Symbiotic in Body Weight, Blood Sugar and Lipemia of Obese Women

Not Applicable
Completed
Conditions
Obesity
Interventions
Dietary Supplement: Probiotic
Dietary Supplement: Symbiotic
Dietary Supplement: Placebo
Registration Number
NCT02505854
Lead Sponsor
Universidade Federal do Rio de Janeiro
Brief Summary

The purpose of this study is to evaluate whether gut microbiota modulation by probiotic and symbiotic contribute with weight loss and improvement of metabolic parameters in women with obesity.

Detailed Description

We will evaluate the effects of probiotic and symbiotic on weigh loss, blood glucose, lipid profile, metabolomic profile, and gut microbiota composition variables in women with obesity.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
32
Inclusion Criteria
  • Women;
  • Adult;
  • Obese (BMI between 30 and 39,9 Kg/m²).
Exclusion Criteria
  • Pregnancy or nursing;
  • Smokers;
  • Drinkers;
  • Use of drug or phytotherapic;
  • Diagnosis of any cronic disease;
  • Daily consumption of yogurt.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
ProbioticProbioticHypocaloric diet associated with capsule containing 1billion UFC Bifidobacterium lactis and sache with 5 g of maltodextrin
SymbioticSymbioticHypocaloric diet associated with capsule containing 1billion UFC Bifidobacterium lactis and sache with 5 g of fructooligosaccharide
PlaceboPlaceboHypocaloric diet associated with capsule containing 50g of gelatin and sache with 5 g of maltodextrin
Primary Outcome Measures
NameTimeMethod
Evaluate the effect of probiotic and symbiotic on weight loss of women obeseBaseline and 60 days and 15 days after the end of the intervention

Determine and evaluate change in body weight and body mass index after intervention.

Secondary Outcome Measures
NameTimeMethod
Effect of probiotic and symbiotic on blood glucoseBaseline, 60 days and 15 days after the end of the intervention

Determine and comparing the blood glucose in plasma before and after intervention

Effect of probiotic and symbiotic on insulin resistanceBaseline and 60 days

Compare insulin resistance through HOMA-IR calculation before and after intervention and evaluate whether the intervention improvement in this parameter.

Effect of probiotic and symbiotic on metabolomic profileBaseline and 60 days

Determine and comparing change in metabolomic profile before and after intervention. The metabolomic profile was evaluated in the serum of blood Bruker DRX 500 MHz equipment (Bruker Spectrospin, Karlsruhe, Germany) generating hydrogen isotope 1 (1H)-NMR spectra, that was compared to database Human Metabolome Data Base.

Effect of probiotic and symbiotic on lipid profileBaseline and 60 days

Compare lipid profile (total cholesterol, low-density lipoprotein, high-density lipoprotein, very low-density lipoprotein, triglyceride) in serum of blood before and after intervention and evaluate whether the intervention improvement in this parameter.

Effect probiotic and symbiotic on blood pressureBaseline,60 days and 15 days after intervention conclusion

Determine blood pressure and evaluate whether the intervention improvement blood pressure.

Effect of probiotic and symbiotic on gut microbiota compositionBaseline, 60 days and 15 days after intervention conclusion

Determine and comparing the amount filos (Fimicutes, Bacteroidetes, Actinobacteria, Verrucomicrobia e Proteobacteria) in gut microbiota and whether the change in microbiota composition continue after stop probiotic and symbiotic intake

Effect of probiotic and symbiotic on stool consitency by Bristol scaleBaseline,60 days and 15 days after intervention conclusion

Determine and comparing the stool consistency by Bristol scale. The Bristol scale is composed of 7 different stool consistency, being 1 and 2 lumpy, 3 and 4 normal, 5 to 7 loose.

Effect of probiotic and symbiotic on gastrointestinal symptonsBaseline,60 days and 15 days after intervention conclusion

Evaluate the presence of gastrointestinal symptoms related to the probiotic and symbiotic. The gastrointestinal symptoms analyzed were abdominal pains, abdominal distension and abdominal discomfort, flatus, borborygmus using the Likert scale of 5 point, which 0 is absence of symptom and 4 is every day.

Trial Locations

Locations (1)

Louise Crovesy de Oliveira

🇧🇷

Rio de Janeiro, Brazil

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