A systems biology approach to RESOLVE the molecular pathology of the hallmarks of patients with metabolic syndrome and its co-morbidities; Hypertriglyceridemia, low HDL-cholesterol and loss of glycemic control.

Completed

Conditions: metabolic syndrome
10018424
10013317

Registration Number: NL-OMON41556

Lead Sponsor: Academisch Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 49

Inclusion Criteria

- Scheduled for bariatric surgery
- 18-65 years of age
- Ability to provide informed consent
- Stable weight 3 months prior to inclusion
- Willingness to stop lipid lowering and antiacid (PPI) medication 4 weeks prior to study measurements.

Exclusion Criteria

- Primary lipid disorder
- Childhood onset obesity (i.e. < 12 years of age)
- Use of exogenous insulin
- All medical and psychiatric conditions except for obesity related diseases.
- Coagulation disorders (prolonged PT, aPTT)
- Uncontrolled hypertension (RR > 150/95 mmHg)
- Renal insufficiency (creatinin > 150 umol/L)
- Excessive alcohol intake (> 14 units/week)
- Pregnancy, females who are breastfeeding
- Contraindication to MR scanning (i.e. pacemaker, metallic foreign body, claustrophobia)

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Differences in tissue expression of genes involved in glucose and lipid<br /><br>metabolism in relation to metabolic fluxes between obese subtypes and between<br /><br>men and women, and the characteristics of the metabolic changes patients<br /><br>undergo during 1 year of extreme weight loss following bariatric surgery.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- Differences in parasympathetic/sympathetic balance and energy expenditure<br /><br>- Differences in liver fat in relation to liver gene expression profiles and<br /><br>metabolic fluxes.<br /><br>- Differences in gut microbiota composition in relation to metabolic fluxes.<br /><br>- The effects of fructose in De Novo Lipogenesis and its relation to other<br /><br>metabolic fluxes.</p><br>