Two-part Safety, Tolerability, Pharmacodynamic and -Kinetic Study of Inhaled AZD8871 in Asthmatic and COPD Subjects

Phase 1

Completed

Conditions: Asthma (Part 1)
COPD (Part 2)

Interventions: Drug: Treatment A, AZD8871 dose A (Part 2)
Drug: Dose 2, AZD8871 100 μg (Part 1)
Drug: Dose 3, AZD8871 300 μg (Part 1)
Drug: Dose 1, AZD8871 50 μg (Part 1)
Drug: Dose 4, AZD8871 600 µg (Part 1)
Drug: Dose 6, AZD8871 1800 μg (Part 1)
Drug: Treatment B, AZD8871 dose B (Part 2)
Drug: Placebo, AZD8871 placebo (Part 1)
Drug: Treatment C, Indacaterol 150 μg (Part 2)
Drug: Dose 5, AZD8871 1200 µg (Part 1)
Drug: Treatment D, Tiotropium 18 μg (Part 2)

Registration Number: NCT02573155

Lead Sponsor: AstraZeneca

Brief Summary: This is a phase I, randomised, placebo-controlled 2-part study to assess the safety, tolerability, pharmacokinetics and pharmacodynamics of AZD8871 delivered by inhalation, in asthmatic and chronic obstructive pulmonary disease (COPD) subjects.

Detailed Description: This study is an integrated Phase I protocol divided into 2 parts.

Part 1: single ascending dose study (6 AZD8871 dose levels) in 16 male subjects with mild asthma. AZD8871 will be administered (by the Genuair® inhaler) under supervision at the study centre, according to the randomisation scheme

Part 2: a 5 treatment period single dose study (of AZD8871 \[two doses\], indacaterol, tiotropium and placebo) in 40 male and non-childbearing female subjects with moderate to severe COPD. Each treatment period will be separated by a washout period of at least 7 days. The primary comparison for bronchodilation will be between AZD8871 doses and placebo.

Recruitment & Eligibility

Status: COMPLETED

Sex: All

Target Recruitment: 134

Inclusion Criteria

Part 1

Subjects who are able and willing to provide written informed consent prior to conducting any study-related procedures, including withdrawal of medications
Adult male subjects aged 18 to 70 years (both inclusive)
Body mass index (BMI) from 18 to 32 kg/m2 at screening
Clinical diagnosis of asthma (according to the Global Initiative for Asthma [GINA] guidelines) for at least 6 months prior to screening
Ability to change current asthma therapy, to discontinue previous prescribed medications after signature of informed consent as per required washout periods
Screening FEV1 value of ≥70% of the predicted normal value
FEV1 reversibility of ≥12% and an absolute increase of at least 200 mL over the baseline value within 30 min after inhalation of 400 µg (4 puffs) of salbutamol via a metered dose inhaler, with spacer device
Subjects using intermittent salbutamol and / or subjects on a stable dose of low dose Inhaled corticosteroid (as defined by the GINA guidelines) at least 4 weeks prior to screening
Predose FEV1 value of first treatment period within the range of ± 20% of the FEV1 measured at screening prior to salbutamol inhalation
No current smokers, no subjects with a smoking history during the last 12 months and no subjects with a total smoking history of more than 10 pack-years
No other relevant pulmonary disease or history of thoracic surgery
Subjects who are otherwise healthy as determined by medical history, physical examination, 12-lead ECG findings
Normal blood pressure (defined as Systolic blood pressure [SBP] between 100 and 140 mmHg for subjects ≤59 years of age and between 100 and 150 mmHg for subjects ≥60 years of age, and Diastolic blood pressure [DBP] between 40 and 90 mmHg) at screening
Subjects whose clinical laboratory test results are not clinically relevant and are acceptable to the Investigator.
Subjects who are negative for hepatitis B surface antigen (HBsAg), hepatitis B core (HBc) antibody (IgM), hepatitis C antibody and human immunodeficiency virus (HIV) I and II antibodies at screening
Subjects who are negative for drugs of abuse and alcohol tests at screening and admission
Subjects able to perform repeatable pulmonary function testing for FEV1 according to the American Thoracic Society (ATS) / European Respiratory Society (ERS) 2005(9) criteria at screening

Inclusion Criteria: Part 2

Adult male and non-childbearing female subjects aged ≥40 years with a clinical diagnosis of stable moderate to severe COPD according to GOLD guidelines at screening
Females must be of non-childbearing potential, confirmed at screening by fulfilling study predefined criteria
Post-salbutamol FEV1 <80% and ≥30% of the predicted normal value and post-salbutamol FEV1 / forced vital capacity (FVC) <70%
BMI < 40 kg/m2 at screening
Ability to change current COPD therapy, to discontinue previous prescribed medications after signature of informed consent as per required washout periods
Current or ex-smokers with a smoking history of ≥10 pack years
No evidence of clinically significant respiratory and / or cardiovascular conditions (e.g. uncontrolled hypertension) or laboratory abnormalities
No other relevant pulmonary disease or history of thoracic surgery
Subjects who are negative for HBsAg, HBc IgM, hepatitis C antibody and HIV I and II antibodies at screening
Subjects who are able and willing to provide written informed consent prior to conducting any study-related procedures, including withdrawal of medications
Medical history must be verified by either a personal physician or medical practitioner as appropriate
Subjects able to perform repeatable pulmonary function testing for FEV1 according to the ATS / ERS 2005(9) criteria at screening

Exclusion Criteria (Part 1 & 2):

Involvement in the planning and/or conduct of the study (applies to both AstraZeneca staff and/or staff at the study site)
Previous enrolment or randomisation of treatment in the present study
Current evidence or recent history of any clinically significant and unstable disease (other than asthma/COPD) or abnormality that could put the subject at risk or could confound the results of the study
Subjects with a surgical history clinically relevant for the purpose of the study
History of malignancy of any organ system, treated or untreated within the past 5 years, with the exception of localised basal cell carcinoma of the skin
Subjects with serious adverse reaction or serious hypersensitivity to Tiotropium (for Part 2 only), Indacaterol (for Part 2 only), or the formulation excipients (eg, lactose) or other drugs in the same pharmacologic class (for Part 1 and Part 2)
Current diagnosis of COPD (for Part 1 only) or history of / or current diagnosis for asthma (for Part 2 only)
Recent history of asthma / COPD exacerbation requiring hospitalisation or need for increased maintenance treatments for asthma / COPD within 6 weeks prior to screening or prior to randomisation
Use of daily oxygen therapy >10 h per day (for Part 2 only)
Use of systemic steroids for respiratory reasons within 6 weeks prior to screening
Lower respiratory tract infection within 6 weeks prior to screening or prior to randomisation
Upper respiratory tract infection requiring antibiotics within 6 weeks prior to screening or prior to randomisation
Current history of tuberculosis, bronchiectasis or other non-specific pulmonary disease
Subject with significant cardiovascular disease that may be vulnerable to cardiovascular instability
QTcF (QT interval corrected, Fridericia formula QT[msec]/RR[s]) interval, >450 ms for males and >470 ms for females at screening or prior to randomisation, or history of long QT syndrome
PR (duration in milliseconds from the beginning of wave P to onset of ventricular depolarisation [Q or R]) interval >200 ms at screening or prior to randomisation (for Part 1 only) Note: 4- 6 hours of ECG rhythm monitoring with telemetry will be performed on Day-1 to identify patients that may have any clinical significant abnormality prior to randomisation. If this occurs, patients should not participate in the study
Subjects with serum potassium concentration < 3.5mmol/l at screening
Subjects with a history of excessive use or abuse of alcohol within the past 2 years
Subjects with a history of drug abuse within the past 2 years
Subjects who are positive for drugs of abuse and alcohol tests at screening and prior to randomisation. Subjects consuming more than 14 (female subjects) or 21 (male subjects) units of alcohol a week
Donation or loss >400 ml of blood and plasma within the previous 3 months prior to screening
Subjects with a significant infection or known inflammatory process at screening or prior to randomisation
Subjects with acute gastrointestinal symptoms at the time of screening or prior to randomisation (eg, nausea, vomiting, diarrhoea, heartburn)
Subjects with an acute infection such as influenza at the time of screening or prior to randomisation
Male subjects who do not agree to follow instructions to avoid pregnancies
Subjects who are not able to adhere to the restrictions on prior and concomitant medications
Subjects who intend to use any concomitant medication not permitted by this protocol or who have not undergone the required washout period for a particular prohibited medication
Subjects who have used any investigational drug within 3 months prior to screening or within the equivalent time of 6 half-lives of receiving the last administration, whichever is longer
Subjects who have received the last dose of investigational product more than 3 months ago but who are on an extended follow-up
Subjects who are unlikely to co-operate with the requirements of the study, or the study center or the subjects who are unwilling or unable to follow the instructions of the principal investigator

Exclusion Criteria

Not provided

Study & Design

Study Type: INTERVENTIONAL

Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Sequence 1, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment A Period 2: Treatment B Period 3: Treatment E Period 4: Treatment C Period 5: Treatment D
Sequence 5, Part 1	Dose 2, AZD8871 100 μg (Part 1)	Period 1: Dose 2 Period 2: Dose 4 Period 3: Dose 6
Sequence 6, Part 1	Dose 6, AZD8871 1800 μg (Part 1)	Period 1: Placebo Period 2: Dose 4 Period 3: Dose 6
Sequence 6, Part 1	Placebo, AZD8871 placebo (Part 1)	Period 1: Placebo Period 2: Dose 4 Period 3: Dose 6
Sequence 1, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment A Period 2: Treatment B Period 3: Treatment E Period 4: Treatment C Period 5: Treatment D
Sequence 2, Part 1	Dose 3, AZD8871 300 μg (Part 1)	Period 1: Placebo Period 2: Dose 3 Period 3: Dose 5
Sequence 7, Part 1	Placebo, AZD8871 placebo (Part 1)	Period 1: Dose 2 Period 2: Placebo Period 3: Dose 6
Sequence 8, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment A Period 2: Treatment E Period 3: Treatment B Period 4: Treatment D Period 5: Treatment C
Sequence 1, Part 1	Dose 1, AZD8871 50 μg (Part 1)	Period 1: Dose 1 Period 2: Dose 3 Period 3: Dose 5
Sequence 1, Part 1	Dose 5, AZD8871 1200 µg (Part 1)	Period 1: Dose 1 Period 2: Dose 3 Period 3: Dose 5
Sequence 3, Part 1	Dose 1, AZD8871 50 μg (Part 1)	Period 1: Dose 1 Period 2: Placebo Period 3: Dose 5
Sequence 3, Part 1	Dose 5, AZD8871 1200 µg (Part 1)	Period 1: Dose 1 Period 2: Placebo Period 3: Dose 5
Sequence 2, Part 1	Dose 5, AZD8871 1200 µg (Part 1)	Period 1: Placebo Period 2: Dose 3 Period 3: Dose 5
Sequence 2, Part 1	Placebo, AZD8871 placebo (Part 1)	Period 1: Placebo Period 2: Dose 3 Period 3: Dose 5
Sequence 5, Part 1	Dose 6, AZD8871 1800 μg (Part 1)	Period 1: Dose 2 Period 2: Dose 4 Period 3: Dose 6
Sequence 6, Part 1	Dose 4, AZD8871 600 µg (Part 1)	Period 1: Placebo Period 2: Dose 4 Period 3: Dose 6
Sequence 1, Part 1	Dose 3, AZD8871 300 μg (Part 1)	Period 1: Dose 1 Period 2: Dose 3 Period 3: Dose 5
Sequence 3, Part 1	Placebo, AZD8871 placebo (Part 1)	Period 1: Dose 1 Period 2: Placebo Period 3: Dose 5
Sequence 4, Part 1	Placebo, AZD8871 placebo (Part 1)	Period 1: Dose 1 Period 2: Dose 3 Period 3: Placebo
Sequence 5, Part 1	Dose 4, AZD8871 600 µg (Part 1)	Period 1: Dose 2 Period 2: Dose 4 Period 3: Dose 6
Sequence 8, Part 1	Dose 2, AZD8871 100 μg (Part 1)	Period 1: Dose 2 Period 2: Dose 4 Period 3: Placebo
Sequence 8, Part 1	Placebo, AZD8871 placebo (Part 1)	Period 1: Dose 2 Period 2: Dose 4 Period 3: Placebo
Sequence 1, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment A Period 2: Treatment B Period 3: Treatment E Period 4: Treatment C Period 5: Treatment D
Sequence 2, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment B Period 2: Treatment C Period 3: Treatment A Period 4: Treatment D Period 5: Treatment E
Sequence 3, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment C Period 2: Treatment D Period 3: Treatment B Period 4: Treatment E Period 5: Treatment A
Sequence 3, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment C Period 2: Treatment D Period 3: Treatment B Period 4: Treatment E Period 5: Treatment A
Sequence 4, Part 1	Dose 1, AZD8871 50 μg (Part 1)	Period 1: Dose 1 Period 2: Dose 3 Period 3: Placebo
Sequence 4, Part 1	Dose 3, AZD8871 300 μg (Part 1)	Period 1: Dose 1 Period 2: Dose 3 Period 3: Placebo
Sequence 7, Part 1	Dose 2, AZD8871 100 μg (Part 1)	Period 1: Dose 2 Period 2: Placebo Period 3: Dose 6
Sequence 7, Part 1	Dose 6, AZD8871 1800 μg (Part 1)	Period 1: Dose 2 Period 2: Placebo Period 3: Dose 6
Sequence 8, Part 1	Dose 4, AZD8871 600 µg (Part 1)	Period 1: Dose 2 Period 2: Dose 4 Period 3: Placebo
Sequence 2, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment B Period 2: Treatment C Period 3: Treatment A Period 4: Treatment D Period 5: Treatment E
Sequence 4, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment D Period 2: Treatment E Period 3: Treatment C Period 4: Treatment A Period 5: Treatment B
Sequence 1, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment A Period 2: Treatment B Period 3: Treatment E Period 4: Treatment C Period 5: Treatment D
Sequence 3, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment C Period 2: Treatment D Period 3: Treatment B Period 4: Treatment E Period 5: Treatment A
Sequence 6, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment D Period 2: Treatment C Period 3: Treatment E Period 4: Treatment B Period 5: Treatment A
Sequence 8, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment A Period 2: Treatment E Period 3: Treatment B Period 4: Treatment D Period 5: Treatment C
Sequence 2, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment B Period 2: Treatment C Period 3: Treatment A Period 4: Treatment D Period 5: Treatment E
Sequence 3, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment C Period 2: Treatment D Period 3: Treatment B Period 4: Treatment E Period 5: Treatment A
Sequence 5, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment E Period 2: Treatment A Period 3: Treatment D Period 4: Treatment B Period 5: Treatment C
Sequence 5, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment E Period 2: Treatment A Period 3: Treatment D Period 4: Treatment B Period 5: Treatment C
Sequence 6, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment D Period 2: Treatment C Period 3: Treatment E Period 4: Treatment B Period 5: Treatment A
Sequence 2, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment B Period 2: Treatment C Period 3: Treatment A Period 4: Treatment D Period 5: Treatment E
Sequence 4, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment D Period 2: Treatment E Period 3: Treatment C Period 4: Treatment A Period 5: Treatment B
Sequence 4, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment D Period 2: Treatment E Period 3: Treatment C Period 4: Treatment A Period 5: Treatment B
Sequence 7, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment E Period 2: Treatment D Period 3: Treatment A Period 4: Treatment C Period 5: Treatment B
Sequence 8, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment A Period 2: Treatment E Period 3: Treatment B Period 4: Treatment D Period 5: Treatment C
Sequence 9, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment B Period 2: Treatment A Period 3: Treatment C Period 4: Treatment E Period 5: Treatment D
Sequence 4, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment D Period 2: Treatment E Period 3: Treatment C Period 4: Treatment A Period 5: Treatment B
Sequence 7, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment E Period 2: Treatment D Period 3: Treatment A Period 4: Treatment C Period 5: Treatment B
Sequence 7, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment E Period 2: Treatment D Period 3: Treatment A Period 4: Treatment C Period 5: Treatment B
Sequence 7, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment E Period 2: Treatment D Period 3: Treatment A Period 4: Treatment C Period 5: Treatment B
Sequence 9, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment B Period 2: Treatment A Period 3: Treatment C Period 4: Treatment E Period 5: Treatment D
Sequence 5, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment E Period 2: Treatment A Period 3: Treatment D Period 4: Treatment B Period 5: Treatment C
Sequence 5, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment E Period 2: Treatment A Period 3: Treatment D Period 4: Treatment B Period 5: Treatment C
Sequence 6, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment D Period 2: Treatment C Period 3: Treatment E Period 4: Treatment B Period 5: Treatment A
Sequence 6, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment D Period 2: Treatment C Period 3: Treatment E Period 4: Treatment B Period 5: Treatment A
Sequence 8, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment A Period 2: Treatment E Period 3: Treatment B Period 4: Treatment D Period 5: Treatment C
Sequence 9, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment B Period 2: Treatment A Period 3: Treatment C Period 4: Treatment E Period 5: Treatment D
Sequence 10, Part 2	Treatment B, AZD8871 dose B (Part 2)	Period 1: Treatment C Period 2: Treatment B Period 3: Treatment D Period 4: Treatment A Period 5: Treatment E
Sequence 10, Part 2	Treatment C, Indacaterol 150 μg (Part 2)	Period 1: Treatment C Period 2: Treatment B Period 3: Treatment D Period 4: Treatment A Period 5: Treatment E
Sequence 10, Part 2	Treatment A, AZD8871 dose A (Part 2)	Period 1: Treatment C Period 2: Treatment B Period 3: Treatment D Period 4: Treatment A Period 5: Treatment E
Sequence 9, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment B Period 2: Treatment A Period 3: Treatment C Period 4: Treatment E Period 5: Treatment D
Sequence 10, Part 2	Treatment D, Tiotropium 18 μg (Part 2)	Period 1: Treatment C Period 2: Treatment B Period 3: Treatment D Period 4: Treatment A Period 5: Treatment E

Primary Outcome Measures

Name	Time	Method
Number of Participants With Clinically Relevant Abnormalities in Blood Pressure	From the time of informed consent up to 36 hours after last dose of IP.	Blood pressure (BP) measurements (diastolic \[DBP\] and systolic BP \[SBP\]) taken after \~5 minutes rest in supine position, at screening, baseline (≤1 hour before IP administration), 10 and 30 minutes, 1, 2, 3, 4, 8, 12 hours (Day 1) and 24 and 36 hours (Day 2) after IP administration. Normal BP at screening: SBP 100-140 mmHg (subjects aged ≤59 years) and 100-150 mmHg (subjects aged ≥60 years), and DBP 40-90 mmHg. Criteria for notable changes in BP: High SBP: (≥180 and increase over baseline (predose) ≥20) or (≥200 and baseline \<200) Low SBP: (≤ 90 and decrease over baseline ≥20) or (≤75 and baseline \>75) High DBP: (≥105 and increase over baseline ≥15) or (≥115 and baseline \<115) Low DBP: (≤50 and decrease over baseline ≥15) or (≤40 and baseline \>40) All out of range values were flagged to the principal investigator who assessed clinical relevance based on medical criteria at individual subject level. Clinically relevant findings were assessed until considered non-clinically relevant.
The Number of Participants With Mild Persistent Asthma (Part 1) and COPD (Part 2) With at Least 1 Treatment-emergent Adverse Event	From the time of informed consent up to 14 (±2) days after the last dose of investigational product. Unresolved AEs were followed up by the investigator for as long as medically indicated.	An adverse event is the development of an undesirable medical condition, or the deterioration of a pre-existing medical condition, following or during exposure to a pharmaceutical product, whether or not considered causally related to the product. An undesirable medical condition can be symptoms, signs, or the abnormal results of laboratory parameters (haematology, blood chemistry, urinalysis, physical examination, 12-lead ECGs and telemetry, and vital signs). AEs were coded using the Medical Dictionary for Regulatory Activities (MedDRA) version 18.1.
Number of Participants With Clinically Relevant Abnormalities in Electrocardiograms (HR, QTcF and Other ECG Parameters).	From the time of informed consent up to 36 hours after last dose of IP.	HR, QTcF and other ECG abnormalities were assessed by local digital 12-lead ECG performed in triplicate at the time points indicated: ECG (Parts 1 and 2) was assessed at Screening, Day 1 baseline, 10 min, 30 min, 1 hour (h), 2 h, 3 h, 4 h, 8 h, 12 h, 24 h, 36 h after dosing. Telemetry (Part 1), assessed with at least 2 lead real time display, was recorded at Day -1 (4-6 hours continuous recording, at the time this was more convenient for the logistics of the unit) and on Day 1 from the time of the IP administration up to at least 24 hours, but if advised by the Investigator or designee, then up to 36 hours after IP administration. Abnormal findings were flagged to the principal investigator who assessed clinical relevance based on medical criteria at individual subject level. Clinically relevant findings were assessed until the abnormality was considered non-clinically relevant.
Number of Participants With Clinically Relevant Abnormalities in Clinical Biochemistry, Hematology and Urinalysis	From the time of informed consent up to 7 days after the last dose of IP.	A composite of clinically relevant abnormalities in clinical biochemistry, hematology and urinalysis laboratory evaluations. Laboratory tests (haematology, blood chemistry, and urinalysis) were performed at screening, at Day -1 (Part 1 only), at 24 hours (Day 2) and at follow-up (7 \[±2\] days) after IP administration. Coagulation was only performed at screening; TSH, T4 only at screening, at Day-1 and at follow-up. Abnormal findings were flagged to the principal investigator who assessed clinical relevance based on medical criteria at individual subject level. Clinically relevant findings were assessed until the abnormality was considered non-clinically relevant.
Change From Baseline in Trough Forced Expiratory Volume in 1 Second (FEV1) on Day 2	Baseline (Day 1) to 36 hours post-dose (Day 2)	Pharmacodynamics of AZD8871 before (-45 min and -15 min pre-dose) and after single dosing of AZD8871 Forced expiratory volume in 1 second (FEV1) on Day 2 (defined as the average of the values 23:00 and 24:00 hours after the morning dose of investigational product)

Secondary Outcome Measures

Name	Time	Method
Cmax of AZD8871 in Parts 1 and 2	Pre-dose, and 5 min, 15 min, 30 min, 45 min, 1 h, 2 h, 3 h, 4 h, 6 h, 8 h, 12 h (Day 1), 24 h, and 36 h (Day 2) post-dose	Cmax (maximum observed plasma drug concentrations) of AZD8871 on Day 1 of each treatment period.
Tmax of AZD8871 in Parts 1 and 2	Pre-dose, and 5 min, 15 min, 30 min, 45 min, 1 h, 2 h, 3 h, 4 h, 6 h, 8 h, 12 h (Day 1), 24 h, and 36 h (Day 2) post-dose	tmax (time to reach maximum concentration) of AZD8871 on Day 1 of each treatment period.
AUC(0-t) of AZD8871 in Parts 1 and 2	Pre-dose, and 5 min, 15 min, 30 min, 45 min, 1 h, 2 h, 3 h, 4 h, 6 h, 8 h, 12 h (Day 1), 24 h, and 36 h (Day 2) post-dose	AUC(0-t) (area under the concentration-time curve from time zero to time of the last quantifiable measurable concentration) of AZD8871 on Day 1 of each treatment period.
AUC(0-24) of AZD8871 in Parts 1 and 2	Pre-dose, and 5 min, 15 min, 30 min, 45 min, 1 h, 2 h, 3 h, 4 h, 6 h, 8 h, 12 h (Day 1), 24 h, and 36 h (Day 2) post-dose	AUC(0-24) (area under the concentration-time curve from zero to 24h) of AZD8871 on Day 1 of each treatment period.