Evaluation of genetic instability and cytotoxicity in professionals who work in surgical center of the veterinary hospital and are exposed to isoflurane anesthetic
Not Applicable
- Conditions
- Occupational ExposureN06.850.460.350.600
- Registration Number
- RBR-3yb4b9
- Lead Sponsor
- Faculdade de Medicina de Botucatu - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ot yet recruiting
- Sex
- Not specified
- Target Recruitment
- Not specified
Inclusion Criteria
Have at least one year of operation in the surgical center; surgery of small and large animals; animal husbandry; animal reproduction; sádios volunteers; both genders; age between 18 and 60 years
Exclusion Criteria
Carriers of malignant disease; infectious diseases: inflammatory; heavy use of alcohol; of illicit drugs; medicines; vitamin supplementation; work in some area with occupational exposure
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method The expected outcome is an increase in DNA damage. The methods of the comet test will be used to evaluate damages and genetic instability by the micronucleus test (MN) method, and cytotoxicity by flow cytometry, where viability and apoptosis and buccal micronucleus will be analyzed, where genomic instability will also be evaluated. Results that achieve a level of 5% will be considered significant. The sample size was calculated based on previous studies with the MN test assuming an expected mean difference between the groups for the buccal MN test around 0.52 and a standard deviation of 0.56, being determined 30 individuals per group with 95% test power.
- Secondary Outcome Measures
Name Time Method Secondary outcomes are not expected