Modern Genetic Methods to Test EPS Samples in Prostatitis Patients

University of British Columbia1 site in 1 country24 target enrollmentDecember 2013

Overview

Brief Summary

Prostatitis is so widespread a disease that affects people from youths to seniors, with approximately a third experiencing a remission of symptoms over a year follow-up. Although the etiology of prostatitis is still not clear, it is mainly thought to be due to infection of bacteria or other microorganisms. Up to now, there is still no research being done on the microbiome (bacterial species) of the prostate. The objective of this study is to look at the etiology of chronic prostatitis(bacteria or non-bacteria prostatitis), mainly focusing on the effect of bacteria in the prostate.

Detailed Description

Prostatitis is so widespread a disease that affects people from youths to seniors, with approximately a third experiencing a remission of symptoms over a year follow-up. Although the etiology of prostatitis is still not clear, it is mainly thought to be due to infection of bacteria or other microorganisms. Traditional techniques to identify bacteria (e.g. on agar petri plates) are limited in what they can identify. Although HMP (Human Microbiome Project) has turned many mysteries into common sense, little is done on the relationship of bacteria in the prostate for prostatitis. Up to now, there is still no research being done on the microbiome (bacterial species) of the prostate. The objective of this study is to look at the etiology of chronic prostatitis (bacteria or non-bacteria prostatitis), mainly focusing on the effect of bacteria in the prostate. Modern sequencing methods, such as 16s rRNA amplification, cloning and sequencing will be used to evaluate the role of bacteria in prostatitis. The basic idea is to survey the microbes present in expressed prostatic fluid using 16s sequencing to compare healthy men and prostatitis patients in a small cohort to see if there are correlations between microbes found and symptoms.

Registry

clinicaltrials.gov

Start Date

December 2013

End Date

December 2023

Last Updated

3 years ago

Study Type

Observational

Sex

Male

Investigators

Sponsor

University of British Columbia

Responsible Party

Principal Investigator

Ben Chew, MD

Associate Professor

University of British Columbia

Eligibility Criteria

Inclusion Criteria

•chronic prostatitis (bacteria or non-bacterial prostatitis) with pelvic pain and LUTS (low urinary tract symptom); diagnosed mainly by NIH-CPSI score. We use NIH-CPSI score 15 as a base line for identifying prostatitis patients:those scored more than 15 will be grouped as prostatitis patients.

Exclusion Criteria

•Inadequate follow-up data
•Other types of prostatits patients
•Patients who in the opinion of the investigators would not be suitable for study
•UTI patients (within 6 months before enrolment)
•Antibiotics (within 6 months before enrolment)
•Urinary tract surgery (within 6 months before enrolment)

Outcomes

Primary Outcomes

Expressed Prostatic Secretions (EPS) for bacterial species in prostatitis patients vs controls

Time Frame: 1 day

Expressed Prostatic Secretions (EPS) will be collected post Digital Rectal Exam (DRE). These biological samples will be tested for bacterial DNA rather than the traditional microbiological culturing techniques. Identification of these bacterial DNA will then be assessed between the groups to determine if prostatitis patients, in fact, do have more bacteria than normal controls.