Metabolic phenotyping of individuals with abdominal obesity and high levels of hepatic fat before and after dietary interventions

Completed

Conditions: algehele gezondheid, metabole gezondheid
abdominal fat
Fatty liver

Registration Number: NL-OMON40205

Lead Sponsor: Wageningen Universiteit

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 110

Inclusion Criteria

Age 40-70y at the time of recruitment
BMI >27kg/m2 or waist circumference >102cm (males) or >88cm (females)

Exclusion Criteria

Hb levels <8.4 mmol/L
Diabetic (normoglycemic according to WHO criteria (OGTT, fasting blood glucose< 7 mmol/L, after 2 hr <11.1 mmol/L)
Daily intake of alcohol of >30g (men) or >20g (women)
Tobacco smoker
Abuse of drugs
Any medical conditions or (metal) devices interfering with or posing a risk for the participant in 1H-MRS/MRI scanning (e.g. claustrophobia, pace maker, surgical screws/pins, artificial joints or heart valves, etc.)
Diagnosed with any long-term medical condition (i.e. cardiovascular disease, gastrointestinal disease, renal dysfunction)
Use of medications known to interfere with glucose or lipid homeostasis (i.e. corticosteroids)
Allergic to fish oil or unwilling to consume fish oil supplements
Unwilling to comply with dietary guidelines
Restricted to a vegetarian dietary regime

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Lipid accumulation in the liver (MRS)<br /><br>Body fat distribution (MRI)<br /><br><br /><br>Physical fitness (VO2 max in a maximal exercise test)<br /><br><br /><br>After a challenge meal:<br /><br>Metabolic plasma markers (cardio-metabolic risk factors, gut function, adipose<br /><br>tissue and muscle health)<br /><br>Peripheral blood mononuclear cells (gene expression patterns)<br /><br>Satiety and well-being (questionnaires)<br /><br>Adipose tissue en muscle biopsies (genome/epigenome determinations)<br /><br>Vascular functions (vascular health, blood pressure, aterial stiffness) </p><br>

Secondary Outcome Measures