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Safety and Efficacy Evaluation of γ-globin Reactivated Autologous Hematopoietic Stem Cells

Not Applicable
Suspended
Conditions
Thalassemia Major
Interventions
Biological: γ-globin reactivated autologous hematopoietic stem cells
Registration Number
NCT05442346
Lead Sponsor
Bioray Laboratories
Brief Summary

This is a single arm, open label, single-dose, phase 1/2 study in up to 5 participants with β-thalassemia major.The study will evaluate the safety and efficacy of the treatment with γ-globin reactivated autologous hematopoietic stem cells in subjects with β-thalassemia major.

Detailed Description

γ-globin reactivated autologous hematopoietic stem cells will be manufactured using Glycosylase Base Editors. Subject participation for this study will be 2 year. Subjects who enroll in this study will be asked to participate in a subsequent long-term follow up study that will monitor the safety and efficacy of the treatment they receive for up to 15 years post-transplant.

Recruitment & Eligibility

Status
SUSPENDED
Sex
All
Target Recruitment
5
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
γ-globin reactivated autologous hematopoietic stem cellsγ-globin reactivated autologous hematopoietic stem cellseach subject will accept one dose of γ-globin reactivated autologous hematopoietic stem cells
Primary Outcome Measures
NameTimeMethod
Proportion of subjects achieving sustained transfusion reduction for at least 3 months (TR3)From 12 months to 24 months post transplant

TR3 was defined as at least a 50% reduction in monthly red blood cell transfusion volume and transfusion frequency compared to baseline for at least 3 months

Proportion of subjects achieving successful neutrophil engraftment within 42 days after BRL-103 infusionFrom 12 months to 24 months post transplant
Time to neutrophil engraftmentFrom 12 months to 24 months post transplant
Time to platelet engraftmentFrom 12 months to 24 months post transplant
Frequency and severity of adverse events through 100 days after BRL-103 InfusionFrom 12 months to 24 months post transplant
Secondary Outcome Measures
NameTimeMethod
Proportion of subjects achieving TR6From 12 months to 24 months post transplant
Proportion of subjects achieving TR12From 12 months to 24 months post transplant
Fetal hemoglobin concentration (pre-transfusion) over timeFrom 12 months to 24 months post transplant
Change in serum ferritin level from baseline over timeFrom 12 months to 24 months post transplant
Proportion of subjects achieving sustained transfusion independence for at least 3 months (TI3)From 12 months to 24 months post transplant

Routine transfusion without disease related and with Hb ≥ 90 g/L for at least 3 months

Proportion of subjects achieving sustained transfusion independence for at least 12 months (TI12)From 12 months to 24 months post transplant
Proportion of alleles with intended genetic modification present in peripheral blood leukocytes over timeFrom 12 months to 24 months post transplant
Proportion of subjects achieving sustained transfusion independence for at least 6 months (TI6)From 12 months to 24 months post transplant
Incidence of transplant related mortality (TRM) within 100 days and within 1 yearFrom 12 months to 24 months post transplant
Frequency, severity, and relationship to BRL-103 of adverse events over two years following BRL-103 infusion.From 12 months to 24 months post transplant
Total hemoglobin concentration (pre-transfusion) over timeFrom 12 months to 24 months post transplant
All-cause mortalityFrom 12 months to 24 months post transplant

Trial Locations

Locations (1)

Shanghai Bioray Laboratories Inc

🇨🇳

Shanghai, Shanghai, China

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