The Effect of Aerobic Training with Vitamin D Supplementation on Cardiomyocytes Gene Expression of Type 2 Diabetic Rats.
Not Applicable
- Conditions
- type 2 diabetes.Type 2 diabetes mellitus
- Registration Number
- IRCT20220512054832N1
- Lead Sponsor
- Razi University of Kermanshah
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Male
- Target Recruitment
- 90
Inclusion Criteria
Age between 2 to 3 months old
To have a minimum of 300 gram of weight
To have type 2 diabetes in diabetic groups
No to have type 2 diabetes in non-diabetic obese groups
Male sex
Exclusion Criteria
Movement disabilities
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Cardiac biomarkers. Timepoint: Before & after the intervention. Method of measurement: Elisa kit (Elabscience Company) with a sensitivity of 18,75 Pg/mL.;Vascular Endothelial Growth Factor B. Timepoint: Before & after the intervention. Method of measurement: Elisa kit (BT-LAB Company) with a sensitivity of 5.01 ng/L.;MicroRNA 1. Timepoint: Before & after intervention. Method of measurement: Polymerase chain reaction (PCR) method using The Corbett Research Rotor-Gene 6000 (QIAGEN company) and The miScript SYBR Green PCR Kit (QIAGEN company) with a sensitivity of 1.55 ng/ml.;Insulin-like growth factor 1. Timepoint: Before & after the intervention. Method of measurement: Polymerase chain reaction (PCR) method using The Corbett Research Rotor-Gene 6000 (QIAGEN company) and The Transcriptor First Strand cDNA Synthesis Kit (Roche company) with a sensitivity of 1.55 ng/ml.
- Secondary Outcome Measures
Name Time Method ipid profile. Timepoint: Before & after the intervention. Method of measurement: Photometry method using Pars Azmoun company kits. The sensitivity of serum level assay kit for LDL Cholesterol, HDL Cholesterol, Triglyceride, and Glucose equals 12.098 mg/dL, 22.714 mg/dL, 35.087 mg/dL, and 25.497 mg/dL, respectively.