Coffee Roasting and Glucose Tolerance

Not Applicable

Completed

• Conditions: Oral Glucose Tolerance

• Registration Number: NCT02417519
• Lead Sponsor: University of Copenhagen

Brief Summary

Chlorogenic acid (CGA) in coffee may lower the postprandial glucose response. CGA is destroyed by dark roasting. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light (LIR) or dark (DAR) roasted coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Plasma glukose and insulin, appetite, and plasma and urine metabolic profiles will be analysed. The primary aim is to investigate whether roasting affects the postprandial glucose area under the curve (AUC).

Detailed Description

Epidemiologic evidence suggests that coffee consumption is associated with a lower risk of type 2 diabetes. Coffee contains caffeine and several other components that may modulate glucose regulation. The chlorogenic acids (CGA) in coffee have been indicated as constituents that may help to normalize the acute glucose response after a carbohydrate challenge. The aim of this study was to investigate whether two coffee beverages that differ in CGA content due to different roasting degrees will affect glucose regulation differently.

In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light roasted (LIR) or dark roasted (DAR) coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Blood samples were drawn at baseline, 30, 60 and 120 min. Differences in glucose and insulin responses and insulin sensitivity index (ISI) were analyzed. The CGA and caffeine contents in the coffees were analyzed using UPLC-MS/MS.

Study Timeline

• Study Start: 2014-04
• Primary Completion: 2014-09
• Study First Submitted: 2015-04-11
• Study First Submitted QC: 2015-04-11
• Study First Posted: 2015-04-15
• Study Completion: 2015-11
• Status Verified: 2017-12
• Last Update Submitted: 2017-12-11
• Last Update Posted: 2017-12-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 11

Inclusion Criteria

Body mass index (BMI) of 18.0-30.0 kg/m2 and with no known diseases

Exclusion Criteria

• systemic infections,
• psychiatric or metabolic disorders,
• known food allergies or intolerances related to the products used in the study (e.g. dairy or gluten),
• ongoing or former drug abuse,
• high intake of alcohol (defined as a weekly intake of >7 units for women and > 14 units for men),
• pregnancy or ongoing planning of pregnancy,
• vegetarianism or veganism,
• participation in other scientific studies during the study period, and
• blood donation during - or in the month leading up to - the study period

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Plasma glucose Area Under the Curve (Glucose AUC)	0-120 min	The area under the plasma glucose concentration curve was calculated from 0-120min after 75g glucose was ingested.

Secondary Outcome Measures

Name	Time	Method
Plasma glucose concentrations	30, 60 and120 min	changes in the glucose concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate.
Serum Insulin Area Under the Curve	0-120 min	Area under the curve (AUC) for serum insulin
ISI(0-120)	0-120min	Matsuda's Insulin sensitivity index
Metabolic profile in plasma	0-24 hours	Metabolic profile of plasma measured in all samples collected before the meal and postprandially from 0-120 min as well as in samples collected up to 24 hrs later.
Serum insulin concentrations	30, 60 and120 min	changes in the insulin concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate.
Metabolic profile in urine	0-24 hours	Metabolic profile of urine samples measured in all samples collected before the meal and postprandially from 0-120 min 120-240min as well as in samples collected up to 24 hrs later.

Trial Locations

Locations (1)

Department of Nutrition, Exercise and Sports, University of Copenhagen; 🇩🇰 Frederiksberg, Denmark