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Physiological Response to Protein and Energy-enhanced Food Products During Winter Military Training

Not Applicable
Completed
Conditions
Malnutrition (Calorie)
Military Operational Stress Reaction
Weight Loss
Muscle Wasting
Interventions
Dietary Supplement: EAA
Dietary Supplement: Energy Dense
Dietary Supplement: Control
Registration Number
NCT05210205
Lead Sponsor
United States Army Research Institute of Environmental Medicine
Brief Summary

Soldiers commonly lose muscle mass during training and combat operations that produce large energy deficits (i.e., calories burned \> calories consumed). Developing new combat ration products that increase energy intake (i.e., energy dense foods) or the amount and quality of protein consumed (i.e., essential amino acid \[EAA\] content) may prevent muscle breakdown and stimulate muscle repair and muscle maintenance during unavoidable energy deficit. The primary objective of this study is to determine the effects of prototype recovery food products that are energy dense or that provide increased amounts of EAAs (anabolic component of dietary protein) on energy balance, whole-body net protein balance, and indices of physiological status during strenuous winter military training.

Detailed Description

Up to 96 Norwegian Soldiers participating in a winter training exercise at the Garrison in Sør-Varanger (GSV) will be enrolled in an approximately 11-day, randomized controlled study. Participants will be randomly assigned at the beginning of the training exercise to groups provided 3 Norwegian Army arctic combat rations and approximately 1500 supplemental calories from food products with increased energy density (EN-DENSE), increased essential amino acids (EAA), or low energy density (CONTROL) each day. Participants will be instructed to consume all of the supplemental food products provided to them and consume the arctic combat rations ad libitum. The effect of consuming EN-DENSE, EAA, or CONTROL food products during strenuous military training on physiological status and recovery will be assessed using dietary analysis, stable isotope methodologies, physical performance measures, gut health analyses, blood sampling, and questionnaires. This design will test the hypothesis that 1) EN-DENSE ration products will attenuate the energy deficit during the training, thereby limiting whole-body protein losses and decrements in physiological status; and 2) the protein-sparing benefit of EAA will limit whole-body protein loss regardless of the energy deficit.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
68
Inclusion Criteria

Male and female Norwegian Soldiers aged 18 years or older participating in the winter training exercise at the Garrison in Sør-Varanger.

Exclusion Criteria
  • Any injury or health condition limiting full participation in the training program.
  • Allergies or intolerance to foods used in the study (including but not limited to lactose intolerance/milk allergy) or vegetarian practices.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
EAA-enhanced food productsEAAConsume approximately 1500 calories of EAA-enhanced food products plus ad libitum consumption of 3 combat rations each day during training.
Energy dense food productsEnergy DenseConsume approximately 1500 calories of energy dense food products plus ad libitum consumption of 3 combat rations each day during training.
Control food productsControlConsume approximately 1500 calories of low energy dense food products plus ad libitum consumption of 3 combat rations each day during training.
Primary Outcome Measures
NameTimeMethod
Protein balance10 hours

Use stable isotope methodologies to measure whole-body protein balance

Energy Expenditure8 days

Use stable isotope methodologies to measure energy expenditure during the training exercise.

Energy intake8 days

Use food logs and collect food wrappers to measure energy intake during the training exercise.

Secondary Outcome Measures
NameTimeMethod
Intestinal Permeability24 hours

Use dual sugar test (i.e., sucralose and erythritol) to measure intestinal permeability

Gut microbiome compositionStudy days -1 and 9

Measure changes in fecal bacterial community, diversity, and relative abundance

Vertical jumpStudy day 0 and 9

Measure changes in physical performance as determined by a vertical jump test

Pull Strength TestStudy day 0 and 9

Measure changes in physical performance as determined by a pull strength test

Trial Locations

Locations (1)

Garrison in Sør-Varanger

🇳🇴

Kirkenes, Norway

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