Effects of an Anthocyanin-rich Blackcurrant Beverage on Cardiovascular Function
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Vascular Stiffness
- Sponsor
- University of Reading
- Enrollment
- 23
- Locations
- 1
- Primary Endpoint
- Change from baseline in platelet function measured by agonist-induced platelet aggregation
- Status
- Completed
- Last Updated
- 10 years ago
Overview
Brief Summary
Regular consumption of fruits and vegetables may improve human health and reduce the risk of chronic diseases, such as heart disease, certain cancers and type 2 diabetes, but the active components and the underlying mechanisms are poorly understood. Berry fruits are abundant in anthocyanins and this study aims to test the hypothesis that ingestion of an anthocyanin-rich blackcurrant beverage will improve markers of cardiovascular health (health of blood vessels, inflammation and platelet function). Further, the study will investigate the anthocyanin bioavailability from the blackcurrant beverage.
Investigators
Professor Parveen Yaqoob, MA, DPhil, RNutr, FAfN
Professor Parveen Yaqoob
University of Reading
Eligibility Criteria
Inclusion Criteria
- •Aged 30-55 years
- •Non-smoker
- •BMI between 20 - 30 kg/m2
- •Generally healthy as established by a 'health and lifestyle' questionnaire and a screening blood sample
- •Blood pressure \< 140/90mmHg
- •Total cholesterol \< 6.2 mmol/L
- •Fasting glucose \< 7.0 mmol/L
Exclusion Criteria
- •Diabetes mellitus
- •Heart problems, stroke, vascular disease
- •Inflammatory disease
- •Kidney, liver, pancreas or gastrointestinal diseases
- •Medication for hyperlipidaemia, hypertension, hypercoagulation, inflammatory conditions
- •Allergies
- •Smokers (social smokers who agree to abstain for 1 month before and during the study not excluded)
- •Taking phytochemical, antioxidant or fish oil supplements (unless willing to stop for the study period)
- •Taking aspirin \> 2 times per month and unwilling to abstain from aspirin ingestion for 14 days prior each study visit
- •History of alcohol misuse
Outcomes
Primary Outcomes
Change from baseline in platelet function measured by agonist-induced platelet aggregation
Time Frame: Acute study: measured at baseline and 2 and 4 h post intervention
Change from baseline in vascular reactivity measured by flow-mediated dilatation (FMD)
Time Frame: Acute study: measured at baseline and 1, 2, 4 and 6 h post intervention
Secondary Outcomes
- Change from baseline in the concentration of polyphenols and their metabolites and degradants in blood and urine samples measured by HPLC-MS/MS(Acute study: plasma measured at baseline and 1, 2, 4, 6 and 24 h post intervention, urine measured at baseline and 1, 2, 4, 6 and 6-24 h post intervention)
- Change from baseline in vascular function measured by digital volume pulse (DVP)(Acute study: measured at baseline and 2, 4 and 6 h post intervention)
- Change from baseline in blood pressure(Acute study: measured at baseline and 1, 2, 4 and 6 h post intervention)
- Change from baseline in the concentration of nitric oxide in plasma measured by ozone-based chemiluminescence(Acute study: measured at baseline and 1, 2, 4 and 6 h post intervention)
- Change from baseline in the concentration of selected cytokines (TNF-a, IL-1b, IL-6, IL-8 and IL-10) in plasma measured using a cytometric bead array kit from BD Biosciences(Acute study: measured at baseline and 1, 2, 4 and 6 h post intervention)
- Change from baseline in platelet function (numbers of circulating micro particles by nano particle tracking analysis)(Acute study: measured at baseline and 1, 2, 4 and 6 h post intervention (urine metabonomics additionally 6-24h))
- Metabonomics on urine and plasma samples measured by nuclear magnetic resonance spectroscopy(Acute study: measured at baseline and 1, 2, 4 and 6 h post intervention (urine metabonomics additionally 6-24h))