Modulation of Metabolic Rate by Inorganic Nitrate

Not Applicable

Completed

• Conditions: Resting Metabolic Rate
• Interventions: Other: Glucose Uptake; Other: Nitrite infusion; Other: Resting metabolic rate

• Registration Number: NCT02022176
• Lead Sponsor: Karolinska Institutet

Brief Summary

The purpose of this study is to determine the effects of sodium nitrate on resting metabolic rate, glucose uptake and formation of reactive oxygen species in humans.

Detailed Description

Not available

Study Timeline

• Study Start: 2008-03
• Primary Completion: 2013-09
• Study Completion: 2013-09
• Study First Submitted: 2013-12-16
• Study First Submitted QC: 2013-12-20
• Study First Posted: 2013-12-27
• Status Verified: 2014-01
• Last Update Submitted: 2014-01-02
• Last Update Posted: 2014-01-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 55

Inclusion Criteria

Healthy volunteers

Exclusion Criteria Chronic medication Vegetarian Nicotine Users Known metabolic disease

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Sodium nitrate	Glucose Uptake	0.1 mmol NaNO3 / kg bodyweight / day for three days.
Sodium nitrate	Resting metabolic rate	0.1 mmol NaNO3 / kg bodyweight / day for three days.
Sodium Chloride	Glucose Uptake	0.1 mmol NaCl / kg bodyweight / day for three days.
Sodium Chloride	Resting metabolic rate	0.1 mmol NaCl / kg bodyweight / day for three days.
Sodium nitrite infusion	Nitrite infusion	Sodium nitrite (NaNO2) at a rate of 1, 10 and 100 nmol kg-1 min-1 (10 minutes per dose) was infused intravenously.
Saline infusion	Nitrite infusion	Saline at a rate corresponding to 1, 10 and 100 nmol NaCl kg-1 min-1 (10 minutes per dose) was infused intravenously.

Primary Outcome Measures

Name	Time	Method
Resting metabolic rate	day 4	Resting metabolic rate, measured after overnight fast using indirect calorimetry

Secondary Outcome Measures

Name	Time	Method
Thyroid hormone status	day 4
Glucose uptake	day 4	Glucose uptake measured with oral glucose tolerance test and euglycemic clamp
Nitrite infusion	30 minutes	Effect on resting metabolic rate when infusing escalating intravenous doses of sodium nitrite or saline solution

Trial Locations

Locations (1)

Karolinska Institutet; 🇸🇪 Stockholm, Sweden