Antibacterial Effectiveness of Two Root Canal Irrigants in Root-filled Teeth With Infection: A Randomized Clinical Trial
Overview
- Phase
- Phase 2
- Intervention
- Not specified
- Conditions
- Periapical Periodontitis
- Sponsor
- University of Oslo
- Enrollment
- 49
- Locations
- 1
- Primary Endpoint
- Periapical radiolucency (PAI- and Friedman-scores)
- Status
- Completed
- Last Updated
- 10 years ago
Overview
Brief Summary
The aim of this randomized clinical trial is to compare the antibacterial effects of 1% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX) during retreatment of teeth with apical periodontitis (AP).
Detailed Description
Root canal-treated teeth with AP were randomly distributed in two groups according to the irrigant used. After removal of the previous filling material, bacteriological samples were taken from the canals before (S1) and after chemomechanical preparation using either NaOCl or CHX (S2), and after calcium hydroxide medication for 2-3 weeks (S3). 16S rRNA gene-based real-time qPCR was performed to detect and quantify total bacteria, Streptococcus species and Enterococcus faecalis.
Investigators
Homan Zandi
DDS
University of Oslo
Eligibility Criteria
Inclusion Criteria
- •Clinical diagnosis of apical periodontitis in a root-filled tooth assessed by radiography.
Exclusion Criteria
- •Presence of gross carious lesions in the diagnosed tooth,
- •Presence of fractures involving the periodontium of the diagnosed tooth,
- •Presence of periodontal pockets more than 4 mm deep in the diagnosed tooth,
- •Diagnosis of diabetes,
- •Diagnosis of HIV infection,
- •Other immunocompromising disease,
- •Antibiotic therapy within the previous 3 months.
Outcomes
Primary Outcomes
Periapical radiolucency (PAI- and Friedman-scores)
Time Frame: 12 months
Secondary Outcomes
- Pyrosequencing of species found before and after the intervention(21 days)
- Bacterial load assessed by qPCR(21 days)