In-situ Cytokines Expression in the CNS in Amyotrophic Lateral Sclerosis (ALS)

Completed

• Conditions: Amyotrophic Lateral Sclerosis

• Registration Number: NCT02582385
• Lead Sponsor: Brugmann University Hospital

Brief Summary

The investigators aim at exploring the differential/topographical in-situ expression of cytokines in the central nervous system (CNS) of patients who died with amyotrophic lateral sclerosis (ALS), using archived histopathology slides and residual paraffin blocks from autopsied cases. Previous studies from the investigators and other groups showed that inflammatory cytokines are implicated in several neurological affections, particularly neurodegenerative conditions. However, in-situ cytokine expression has never been studied so far in ALS. The investigators wanted to see if these neuro-mediators are involved in the neuromolecular chain/cascade underlying ALS.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-10
• Primary Completion: 2015-02
• Study Completion: 2015-02
• Status Verified: 2015-10
• Study First Submitted: 2015-10-20
• Study First Submitted QC: 2015-10-20
• Last Update Submitted: 2015-10-20
• Study First Posted: 2015-10-21
• Last Update Posted: 2015-10-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 5

Inclusion Criteria

• patients who died with amyotrophic lateral sclerosis, having been autopsied.

Exclusion Criteria

• N/A

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
in situ cytokine detection: interleukin 18	retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005	IHC detection on histopathology sections of paraffin embedded brain tissue.
in situ cytokine detection: interleukin 1	retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005	IHC detection on histopathology sections of paraffin embedded brain tissue.
in situ cytokine detection: interleukin 6	retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005	IHC detection on histopathology sections of paraffin embedded brain tissue.
in situ cytokine detection:TNF-a	retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005	IHC detection on histopathology sections of paraffin embedded brain tissue.

Trial Locations

Locations (1)

CHU Brugmann; 🇧🇪 Brussels, Belgium