Antioxidants Vitamins and Muscle Damage

Not Applicable

Completed

• Conditions: Oxidative Stress
• Interventions: Dietary Supplement: Placebo

• Registration Number: NCT05127928
• Lead Sponsor: Universidad Europea de Madrid

Brief Summary

Determine if acute supplementation with antioxidant vitamins (vitamin C and vitamin E) influences the muscle damage and fatigue caused after an acute session of eccentric training.

Detailed Description

A randomized, single-blind, placebo-control parallel trial will be conducted. In a previous session volunteers will be interviewed to determine if they meet the inclusion criteria.

Participants should avoid ingesting of foods that contain high amounts of antioxidant compounds: more than two juices of fruits, more than four cups of coffee or tea. Juices with high antioxidant content should be avoided. Alcoholic beverages should also be avoided. Participants will be divided in to groups and will performance one resistance exercise sessions. Prior to exercise, one group will intake supplementation VitC (1000 mg) + VitE (235 mg) and the other supplementation with placebo.

The following sequence will be performed in each session: 1) baseline assessment (analysis of the composition body, blood draw and parameters of performance) prior to exercise session ; 2) administration of vitamin or placebo supplementation 2 hours before exercise session ; 3) post-exercise assessments (blood biomarkers and performance parameters) immediately after the session and after 24 hours. DOMS will also be assessed 48 h and 72 h after training. The training session implemented will be designed by a Sport Scientific

Study Timeline

• Study First Submitted: 2021-06-15
• Study First Submitted QC: 2021-11-08
• Study First Posted: 2021-11-19
• Study Start: 2022-02-10
• Primary Completion: 2022-02-15
• Study Completion: 2022-03-06
• Status Verified: 2022-04
• Last Update Submitted: 2022-04-04
• Last Update Posted: 2022-04-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

• Healthy
• Physically active

Exclusion Criteria

• Injuries
• Supplementation or drugs
• Smokers

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Placebo	Placebo	Placebo before exercise

Primary Outcome Measures

Name	Time	Method
Percentage of body fat and muscle mass	Prior to the intervention	Determination of body composition using skinfolds measurements
Jump height in a Counter Movement Jump	24 h after exercise	Jump height (cm) in a counter movement jump measured with Octojump Platform
Concentration of blood lactate	2 hours before exercise	Capillary blood lactate with Lactate Pro 2
Blood malondialdehyde (MDA)	24 hours after exercise	MDA will be analyzed from a blood sample
Contact time in a Drop Jump	Inmediately after exercise	Capillary blood lactate evaluated with Lactate Pro 2
DOMS	24 hours post exercise	Delayed onset muscle soreness. This outcome will be measure with a 10-point visual analogic scale (0= no pain; 10 = worst possible pain)
Blood creatine quinase (CK)	24 hours after exercise	CK will be analyzed from a blood sample
Jump Height in a squat Jump	2 hours prior to exercise	Jump height (cm) in a squat jump with Octojump Platform measured with Octojump Platform
Jump Height in a Squat Jump	Inmediately after exercise	Jump Height (cm) in a Squat Jump measured with Octojump Platform
Jump Height in a Drop Jump	24 hours after exercise	Jump height (cm) measured in different drop jumps with Octojump Platform

Trial Locations

Locations (1)

Universidad Europea de Madrid; 🇪🇸 Madrid, Spain