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The effect of anterior cruciate ligament injury on skeletal muscle function, contractile proteins and inflammatio

Not Applicable
Completed
Conditions
Anterior cruciate ligament injury
Musculoskeletal - Other muscular and skeletal disorders
Registration Number
ACTRN12613000157774
Lead Sponsor
Victoria University
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
All
Target Recruitment
12
Inclusion Criteria

ACL group:
1. Scheduled for ACL reconstructive surgery.
2. Between 18-35 years of age
3. BMI <30

Control Group:
1. Free from lower limb injury
2. Between 18-35 years of age
3. BMI <30

Exclusion Criteria

ACL group:
Not between 18-35 years of age.
Any metabolic or cardiovascular diseases which impact on physical activity prior to ACL injury.

Control group
Not between 18-35 years of age
Any metabolic or cardiovascular diseases which impact on physical activity levels
Recent lower limb joint injury

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Muscle Na+,K+ ATPase transport protein content. Measured using radioactive labelled ouabain binding site content for total functional Na+,K+ ATPase content, and western blotting for indivividual isoform content.[Immediately before the commencement of ACL reconstructive surgery.];Muscle function (strength and balance). Isometric strength measured using a portable force transducer strapped to a chair at 90 degree knee flexion.<br><br>Balance will be assessed using a force platform which can calculate the average velocity of the participant's centre of pressure. [Approximately 1-2 weeks before ACL reconstructive surgery.]
Secondary Outcome Measures
NameTimeMethod
Muscle inflammatory factors (IL-6, TNF alpha). This will be assessed using a Multiplex suspension array system (Bio-Rad). [Immediately before ACL reconstructive surgery.]
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