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A Study to Determine Iatrogenic Hyperinsulinemia's Contribution to Insulin Resistance and Endothelial Dysfunction in Type 1 Diabetes

Not Applicable
Completed
Conditions
Diabetes Mellitus, Type 1
Interventions
Other: Standard Carb Diet
Other: Low Carb Diet
Registration Number
NCT04118374
Lead Sponsor
Vanderbilt University Medical Center
Brief Summary

The investigators will test the hypothesis that reducing insulin doses using a low carbohydrate diet (LCD) will be associated with with improved insulin sensitivity (Aim 1) and blood vessel health (Aim 2).

Detailed Description

Insulin resistance (IR) is consistently found in patients with type 1 diabetes (T1DM) and pathophysiologically links T1DM with atherosclerotic disease. IR and nascent atherosclerosis, as characterized by endothelial dysfunction, are present early in T1DM. Although atherosclerosis leads to cardiovascular disease (CVD)-the predominant cause of death in T1DM-the early cardiometabolic processes driving atherosclerosis are not currently well-characterized. My overarching hypothesis is that IR and endothelial dysfunction in T1DM are, in part, iatrogenic, occurring as a function of nonphysiologic insulin delivery.

Previous research shows IR in T1DM is closely related to iatrogenic hyperinsulinemia. Iatrogenic hyperinsulinemia in T1DM results from injecting insulin into subcutaneous tissue rather than delivering insulin more physiologically into the hepatic portal vein. Hyperinsulinemia, per se, is closely linked with IR and independently predicts CVD in diabetic and nondiabetic populations. Thus, peripheral insulin delivery brings about unintended adverse cardiometabolic consequences in T1DM. The investigators propose a practical intervention to diminish iatrogenic hyperinsulinemia and thereby mitigate CVD risk. The investigators hypothesize that a reduction in iatrogenic hyperinsulinemia brought about by a low carbohydrate diet (LCD) will independently correlate with improved insulin sensitivity (Aim 1) and endothelial function (Aim 2).

In this pilot study, the investigators will mechanistically dissect the contribution of iatrogenic hyperinsulinemia to IR and endothelial dysfunction in 8 adults with T1DM using a crossover study of LCD vs. standard carbohydrate diet (SCD) to experimentally modify hyperinsulinemia. The investigators will quantify insulin sensitivity using hyperinsulinemic, euglycemic clamps and measure endothelium-dependent flow mediated vasodilation using high-resolution ultrasound.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
12
Inclusion Criteria
  • Age: 18-60
  • HbA1c: 5.6-9.0%
  • Insulin delivery: must be on an insulin pump
  • Glucose Monitor: must use a continuous glucose monitor (CGM)
  • BMI 18-33 kg/m^2
  • Body Mass >/= 50 kg ( 110 lbs)
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Exclusion Criteria
  • severe hypoglycemia : >/= 1 episode in the past 3 months
  • diabetes comorbidities (>= 1 trip to emergency department for poor glucose control in the past 6 months,
  • New York Heart Association Class II-IV cardiac functional status
  • SBP > 140 and DBP > 100 mmHg,
  • eGFR by MDRD equation of <60 mL/min/1.73m^2
  • AST or ALT > 2.5 times the upper limit of normal
  • HCT <35%

medications

  • any antioxidant vitamin supplement (<2 weeks before STUDY visit)
  • any systemic glucocorticoid
  • any antipsychotic
  • atenolol, metoprolol, propranolol
  • niacin
  • any thiazide diuretic
  • any OCP with > 35 mcg ethinyl estradiol,
  • growth hormone
  • any immunosuppressant
  • any antihypertensive
  • any antihyperlipidemic

other:

  • pregnancy
  • Tanner stage < 5
  • peri or postmenopausal woman
  • active smoker
  • gluten-free diet requirement

Additional exclusion criteria for T1DM subjects

  • any diabetes medication except insulin
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Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Low Carb Diet then Standard Carb DietLow Carb Diet-
Standard Carb Diet then Low Carb DietLow Carb Diet-
Standard Carb Diet then Low Carb DietStandard Carb Diet-
Low Carb Diet then Standard Carb DietStandard Carb Diet-
Primary Outcome Measures
NameTimeMethod
Change in insulin sensitivity - diet 1baseline to 1 week

Change in insulin sensitivity based on diet

Change in insulin sensitivity - diet 2baseline to 1 week

Change in insulin sensitivity based on diet

Change in endothelial function - diet 1baseline to 1 week

will quantify brachial artery, endothelium-dependent flow-mediated vasodilation using high-resolution ultrasound

Change in endothelial function - diet 2baseline to 1 week

will quantify brachial artery, endothelium-dependent flow-mediated vasodilation using high-resolution ultrasound

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Vanderbilt University Medical Center

🇺🇸

Nashville, Tennessee, United States

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