Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome

• Conditions: Irritable Bowel Syndrome; Colonic Diseases; Colonic Diseases, Functional; Digestive System Diseases; Gastrointestinal Diseases; Intestinal Diseases

• Registration Number: NCT01028898
• Lead Sponsor: Chinese PLA General Hospital

Brief Summary

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Detailed Description

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Study Timeline

• Study Start: 2006-01
• Status Verified: 2006-12
• Study First Submitted: 2009-12-08
• Study First Submitted QC: 2009-12-08
• Study First Posted: 2009-12-09
• Last Update Submitted: 2011-02-10
• Last Update Posted: 2011-02-11
• Primary Completion: 2011-08
• Study Completion: 2012-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 180

Inclusion Criteria

• Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

Exclusion Criteria

• Patients with organic illnesses or a history of major abdominal surgery
• Major psychotic episode, mental retardation or dementia
• Pregnant women and lactational women
• Postinfectious IBS, PI-IBS

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified