Association Between Mitochondrial DNA Content and Risk of Coronary Heart Disease

Completed

• Conditions: Coronary Heart Disease

• Registration Number: NCT02500823
• Lead Sponsor: Air Force Military Medical University, China

Brief Summary

Compelling epidemiological evidence indicates that alterations of mitochondrial DNA, including mutations and abnormal content of mitochondrial DNA (mtDNA), are associated with the initiation and development of cardiovascular disease. This study was undertaken to investigate whether mtDNA copy number in peripheral blood cells could be used as a risk predictor for coronary heart disease.

Detailed Description

Not available

Study Timeline

• Study Start: 2015-04
• Study First Submitted: 2015-07-15
• Study First Submitted QC: 2015-07-16
• Study First Posted: 2015-07-17
• Primary Completion: 2015-08
• Study Completion: 2015-11
• Status Verified: 2016-05
• Last Update Submitted: 2016-05-14
• Last Update Posted: 2016-05-17

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 400

Inclusion Criteria

1. History of documented myocardial infarction;
2. Prior coronary revascularization intervention (coronary artery bypass graft surgery or percutaneous coronary intervention);
3. The presence of ≥ 50% stenosis in one or more coronary arteries identified during cardiac catheterization;

Exclusion Criteria

1. History of malignancy or end-stage renal disease;
2. Blood transfusion within one month or prior bone marrow transplantation
3. Patients who reluctant to sign informed consent

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Relative copy number of mitochondrial DNA	From date of percutaneous coronary intervention until the date of discharging from hospital, assessed up to 5 days	The ratio of mitochondrial DNA copy number to hemoglobin copy number was calculated for each sample from standard curves. After that, the ratio for each sample was normalized to a calibrator DNA in order to standardize between different runs, and then defined as the measurement of relative mtDNA copy number.Relative copy number of Mitochondrial DNA were measured in CHD patients in comparison to control group.

Trial Locations

Locations (1)

Xijing Hospital; 🇨🇳 Xi'an, Shaanxi, China