Mitochondrial Function in Circulating Cells and Muscle Tissue

Completed

• Conditions: Obesity; Healthy; Insulin Sensitivity; Women

• Registration Number: NCT02700698
• Lead Sponsor: University of Arkansas

Brief Summary

The main goal of this project is to determine whether mitochondrial function in circulating cells is related to that measured in the muscle fibers of the same subjects.

Detailed Description

Data from human and animal models strongly suggests that reduced tissue mitochondrial function can be an early marker for detection of dysfunctional metabolism. It is unclear if tissue mitochondrial dysfunction can be detected using less invasive methodology. In this project, the investigators plan to study mitochondrial bioenergetics in circulating cells and paired muscle biopsies in two metabolic disparate groups of women; healthy lean vs. overweight/obese with/without insulin resistant women of similar age. Women of similar age that are either healthy lean or overweight/obese with/without insulin resistance will be recruited to participate in one study that includes a blood draw and a muscle biopsy. Mitochondrial function in circulating blood cells and muscle tissue will be determined. The association between circulating blood cells and muscle tissue mitochondria respiration will be determined for the two groups combined, and differences between groups will also be investigated.

Study Timeline

• Study First Submitted: 2016-02-16
• Study First Submitted QC: 2016-03-01
• Study First Posted: 2016-03-07
• Study Start: 2016-09
• Primary Completion: 2018-04-26
• Study Completion: 2018-05-01
• Results First Submitted: 2021-09-02
• Status Verified: 2021-10
• Results First Submitted QC: 2021-10-26
• Last Update Submitted: 2021-10-26
• Results First Posted: 2021-12-01
• Last Update Posted: 2021-12-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 35

Inclusion Criteria

• Women
• 25-35 years of age
• Either healthy lean (BMI<25) or insulin resistant and obese (BMI >30)

Exclusion Criteria

• Medication that can affect study outcomes, as determined by the principal investigator, including anti-diabetic medication
• Chronic diseases, acute inflammation, chronic renal failure (glomerular filtration rate <45), or heart problems

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women	During the visit, approximately 0.5 days	Skeletal muscle (m.vastus lateralis) and peripheral blood mononuclear cell (PBMC) mitochondrial respiration were determined in response to substrate inhibitor coupling protocols using high resolution respirometry.; From this, mitochondrial respiration in leak and coupled states, and reserve respiratory capacity were determined in mitochondria from skeletal muscle and from PBMCs. It was determined whether mitochondrial function in circulating cells is related to that measured in the muscle tissue of the same subjects.

Trial Locations

Locations (2)

UAMS; 🇺🇸 Little Rock, Arkansas, United States

Arkansas Children's Nutrition Center; 🇺🇸 Little Rock, Arkansas, United States