The Effect of Leukocyte Dna mEthylation and micRoBIOME Diversity on Host Defense Mechanisms During Community-acquired Pneumonia
试验速览
- 阶段
- 不适用
- 入组人数
- 231
- 试验地点
- 1
- 主要终点
- Alterations in leukocyte DNA methylation
研究概览
简要总结
Community-acquired pneumonia (CAP) represents a major health care problem and mortality and morbidity associated with severe pneumonia remain considerable, despite state of the art care.
While the role of altered DNA methylation in cancer has been widely studied, knowledge of its impact on antibacterial defense is highly limited. In addition, recent preclinical studies showed that the gut and respiratory microbiota contributes to host defense against bacterial pneumonia.
This study aims to explore a completely novel research area linking the extent of DNA methylation in blood leukocyte (monocytes and neutrophils) and function of gut and respiratory microbiota on the influence of innate immune responses to and host defense against CAP
详细描述
Rationale:
Community-acquired pneumonia (CAP) represents a major health care problem and mortality and morbidity associated with severe pneumonia remain considerable, despite state of the art care.
While the role of altered DNA methylation in cancer has been widely studied, knowledge of its impact on antibacterial defense is highly limited. In addition, recent preclinical studies showed that the gut and respiratory microbiota contributes to host defense against bacterial pneumonia.
This study aims to explore a completely novel research area linking the extent of DNA methylation in blood leukocyte (monocytes and neutrophils) and function of gut and respiratory microbiota on the influence of innate immune responses to and host defense against CAP.
Primary Objectives:
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Clinical suspicion of a new episode of acute respiratory tract infection
- •Primary reason for presentation is clinical suspicion of a new episode of acute respiratory infection; admission to the hospital is NOT a requirement
- •Evident new or progressive infiltrate, consolidation, cavitation, or pleural effusion on the chest X ray or CT scan made for diagnostic (non-research) purposes
- •Onset of the following symptoms within the last 7 days:
- •At least one respiratory symptom (cough, sore throat, runny or congested nose, dyspnea)
- •At least one systemic symptom (fever, headache, muscle ache, sweats or chills or tiredness).
排除标准
- •Patient lacks capacity to provide informed consent
- •No informed consent is provided by patient
- •Patient has been transferred from another hospital
- •Patient is enrolled in an interventional clinical study of an anti-infective or immunomodulatory therapy
- •Patient has received any type of oral or systemic antibiotics for more than 48 hours prior to hospital presentation.
结局指标
主要结局
Alterations in leukocyte DNA methylation
时间窗: day 0
次要结局
- Composition and function of the gut and nasopharyngeal microbiota(day 28)
- Alterations in leukocyte DNA methylation(day 28)
研究者
T. van der Poll
prof. dr.
Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)