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Oral Pomegranate Extract on the Microbiome and Skin Biophysical Properties

Not Applicable
Completed
Conditions
Human Microbiome
Interventions
Other: Placebo
Dietary Supplement: Pomella
Registration Number
NCT04596722
Lead Sponsor
Integrative Skin Science and Research
Brief Summary

Pomegranate extract (Pomella) is well known for its antioxidant properties due to its phenolic compounds. It has also been shown to increase the amount of short chain fatty acid producing Lactobacillus and Bifidobacteria genera. Short chain fatty acids are thought to have an anti-inflammatory effect on the sebaceous glands. Previous studies have concluded that pomegranate extract may act as a prebiotic in the body and subsequently increasing the gastrointestinal microbial diversity and by producing short chain fatty acids that may have systemic beneficial effects especially on the skin.

Therefore, the aim of this study is to assess how Pomella alters the gut microbiome and the blood level of short chain fatty acids in healthy subjects.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
18
Inclusion Criteria
  1. Subjects aged 25-55
  2. Must be willing to comply with all protocol requirements
  3. Must be willing to have flash photo facial images taken with the imaging systems
  4. Males must be willing to shave any facial hair
Exclusion Criteria
  1. Any systemic or antibiotics (injected or oral) within 6 months of starting study
  2. Any topical antibiotic or benzoyl peroxide within 1 month of starting study or any subject unwilling to refrain from washout of topical antibacterial or benzoyl peroxide ingredient.
  3. No intake of alcohol or tea throughout the duration of the study.
  4. No more than 1 8 oz cup of coffee per week throughout the duration of the study.
  5. No more than 1 cup of berries (strawberries, blueberries, raspberries, blackberries, etc.) per week throughout the duration of the study.
  6. No intake of pomegranate, or pomegranate-containing drinks throughout the duration of the study.
  7. No intake of chocolate (in any form) throughout the duration of the study.
  8. No more than 1 cup of fermented dairy products per week throughout the duration of the study.
  9. Any oral probiotic or prebiotic supplementation within past 1 month
  10. Subjects must have no history of malignancy or cancer or diagnosis of gastrointestinal inflammatory diseases, no history or diagnosis of epilepsy, no history or diagnosis of immunologic or infectious disease (e.g. hepatitis, tuberculosis, HIV or AIDS, lupus, rheumatoid arthritis)
  11. Has a condition or is on medication the investigator and/or designee believe could jeopardize the safety of the subject, interfere with the evaluation, or confound the interpretation of the study results
  12. Women who have been pregnant in the last three months, are pregnant, preparing to be pregnant or lactating.
  13. Is participating in a concurrent clinical research study or has participated in an acne or other facial study at this or any other facility in the past 4 weeks
  14. Those with BMI higher than 35 kg/m²
  15. Commencement of a new diet (such as the ketogenic diet) or supplements within the 1 month prior to initiating participation, at the discretion of the investigator.
  16. Use of medications that alter blood lipids, such as statins and anti-hyperlipidemic medications
  17. Is participating in or has participated in a facial study at this or any other facility in the past 4 weeks. Participation in survey-based studies are approved at the discretion of the investigator.
  18. Has a skin disease on face that will interfere with image collection and assessment in the opinion of the investigator
  19. Refusal to shave or remove facial hair that may interfere with image collection and assessment.
  20. Persons unwilling to avoid the following during the 4 weeks prior and during the duration of the study: self-tanning, spa tanning, sun tanning, or artificial tanning.
  21. Planned vacation to sunny destination with the intention of sun tanning during the duration of the study.
  22. Persons who regularly undergo sauna treatments (dry or wet) or who swim daily.
  23. Known allergy or irritation to the supplement or facial products utilized in the study
  24. Current tobacco smokers, OR those that have smoked tobacco over the past year, OR a 5 year-pack year history of smoking tobacco
  25. Prisoners
  26. Adults unable to consent
  27. Vegans

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlacebooral placebo taken by mouth once per day
Assigned InterventionsPomellaoral active pomella taken by mouth once per day
Primary Outcome Measures
NameTimeMethod
Shift in Gut Microbiome Diversity4 weeks

Stool is collected and analyzed for species of bacteria through nucleic acid sequencing.

Secondary Outcome Measures
NameTimeMethod
Change in Transepidermal Water Loss4 weeks

Delfin Vapometer 0-300g/m\^2h; Delfin MoisturemeterSC 0-150

Safety Assessment for GI Distress4 weeks

Subjective GI based Questionnaire that will assess for distress (one combined metric that accounts for stooling, gas, and discomfort)

Change in Skin Brightness - Device based4 weeks

SkinColorCatch colorimeter RGB range: 25-246 per channel

Change in skin brightness - image based4 weeks

BTBP Clarity Mini 3D Camera

Gut Microbiome Changes- Abundance and Presence of Short Chain Fatty Acid producing Bacteria4 weeks

Stool is collected and analyzed for species of bacteria through nucleic acid sequencing.

Change in Sebum Excretion Rate4 weeks

Delfin Sebumeter: 0-150 micrograms/cm\^2

Shift in the Blood Short Chain Fatty Acid Levels4 weeks

Short chain fatty acids are measured from a venipuncture

Change in the Appearance of Facial Redness and Facial Skin Tone4 weeks

SkinColorCatch colorimeter RGB range: 25-246 per channel; BTBP Clarity Mini 3D_V5 camera

Trial Locations

Locations (1)

Integrative Skin Science and Research

🇺🇸

Sacramento, California, United States

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