Urine Prostaglandin-M(PGE-M) in Ovarian Cancer
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 7
- 主要终点
- Prostaglandin E2 metabolite (PGE-M) levels in 24-hour urine samples
研究概览
简要总结
RATIONALE: Measuring the amount of prostaglandin metabolite in urine samples and studying tissue samples in the laboratory from patients with ovarian cancer may help doctors identify and learn more about biomarkers related to ovarian cancer and may help the study of cancer in the future.
PURPOSE: This laboratory study is measuring the amount of prostaglandin metabolite in urine samples from patients with ovarian cancer.
详细描述
OBJECTIVES:
- Determine the level of prostaglandin E_2 metabolite (PGE-M) in 24-hour urine samples collected prior to surgery or chemotherapy from patients with ovarian cancer.
- Determine cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) expression by western blot and immunohistochemistry (IHC) in ovarian tumor tissue samples and matched normal ovarian tissue samples, if available, from these patients.
- Correlate urine PGE-M levels with corresponding tissue COX-1 and COX-2 levels in urine and tissue samples from these patients.
OUTLINE: This is a pilot study.
Patients submit 24-hour urine specimens prior to surgery for evaluation of urinary levels of prostaglandin E_2 metabolite (PGE-M).
Patients also undergo assessment of tissue obtained at the time of surgery, including ovarian tumor tissue, normal surface ovarian epithelium tissue (if available), and fixed tissue embedded in a paraffin block. Tissue samples are analyzed by western blot and IHC for cyclooxygenase-1 and cyclooxygenase-2 expression.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 17 Years 至 —（Child, Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Diagnosis of known or suspected ovarian cancer
- •Age greater than 17 years
排除标准
- •Pregnant or nursing
- •Taking steroids or non-steroidal anti-inflammatory drugs (NSAIDs) on a regular basis.
- •PRIOR CONCURRENT THERAPY:
- •No concurrent chronic use of steroids or NSAIDs
结局指标
主要结局
Prostaglandin E2 metabolite (PGE-M) levels in 24-hour urine samples
时间窗: Pre-surgical or chemotherapeutic treatment, over a 24-hour duration
Patients will be collect their urine for 24 hours before they undergo any surgery or chemotherapy. Researchers will measure the amount of PGE-M in the urine.
次要结局
- COX-1 and COX-2 expression in ovarian tumor tissue and non-malignant tissue(Day of surgery)
- Correlation of urine PGE-M levels with tissue COX-1 and COX-2 levels(After patients' surgery)
研究者
Marta Crispens, MD
Associate Professor; Gynecological Oncologist
Vanderbilt-Ingram Cancer Center