Mechanisms for Laser-Induced Rejuvenation

Not Applicable

Not yet recruiting

• Conditions: Skin Cancer
• Interventions: Device: Fractionated Laser Resurfacing

• Registration Number: NCT06489301
• Lead Sponsor: Wright State University

Brief Summary

It has shown that laser wounding of geriatric skin protects it against skin cancer. The purpose of this study is to determine the origin of cells called fibroblasts which are present after treatment with Fractionated Laser Resurfacing (FLR). These cells restore a youth-like ultraviolet B (UVB) response, but where they come from is currently unknown. The study recruits subjects who are scheduled to undergo abdominoplasties to investigate the origin of those cells stimulated by laser treatment.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2024-05-21
• Study First Submitted QC: 2024-06-28
• Study First Posted: 2024-07-05
• Status Verified: 2025-03
• Last Update Submitted: 2025-03-24
• Last Update Posted: 2025-03-25
• Study Start: 2025-07
• Primary Completion: 2028-12
• Study Completion: 2028-12

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• Adult males and females 18 and older
• Able to comprehend procedures and risks
• Planning to undergo abdominoplasty surgery
• Minimum amount of skin to be removed is 50 x 20cm

Exclusion Criteria

• Medical history of not well-controlled diabetes. This is defined as a subject who needs high doses of insulin, recent history of hospitalization for diabetes-related complications, or poor wound health as evidenced by diabetic ulcers. Subjects who have been deemed healthy enough for abdominoplasty surgery would tend to suggest that their diabetes should not be an issue. However, if the subjects have diabetic complications as outlined above, they could affect the wound healing which is the object of this protocol so they would be excluded.
• Pregnant or nursing
• History of pathologically abnormal wound healing such as keloidal scars
• Existing tattoos on the abdomen that are so extensive that they could interfere with the study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Fractionated Laser Resurfacing	Fractionated Laser Resurfacing	At 14 days prior to scheduled surgery a 5x5cm square on the left abdomen within the region of skin to be excised and treated with fractionated laser resurfacing. Then at 7 days prior to scheduled surgery a 5 cm diameter circle on the right abdomen within the region of skin to be excised, at least 10 cm away from the square, is treated with fractionated laser resurfacing.

Primary Outcome Measures

Name	Time	Method
To test if possible cells transitioning from monocytes to fibroblasts are found in the top part of the dermis (papillary dermis).	2 weeks prior to skin removal and 1 week prior to skin removal	Using tissue RNA-seq, PI will define if co-expressing cells are in the papillary dermis.
To test if the new fibroblasts that develop in response to fractionated laser resurfacing (FLR) wounding are derived from blood-derived monocytes.	2 weeks prior to skin removal and 1 week prior to skin removal	Identify cells in the skin that have been wounded by FLR at 2 weeks and 1 week previously, for cells that co-express markers for both fibroblasts and monocytes by using single cell RNA-sequencing

Secondary Outcome Measures

Name	Time	Method
To define the locations of the types of cells populating in the skin following fractionated laser resurfacing (FLR) wounding	2 weeks prior to skin removal and 1 week prior to skin removal	Define the locations of the different cell types following FLR wounding at 2 weeks and 1 week prior to skin removal by using tissue RNA-seq.
To define the number and types of cells populating the skin following fractionated laser resurfacing (FLR) wounding	2 weeks prior to skin removal and 1 week prior to skin removal	Define the number and types of cells following FLR wounding at 2 weeks and 1 week prior to skin removal by using single-cell RNA-seq.
To define the locations of the number of cells populating in the skin following fractionated laser resurfacing (FLR) wounding	2 weeks prior to skin removal and 1 week prior to skin removal	Define the locations of the number cell following FLR wounding at 2 weeks and 1 week prior to skin removal by using tissue RNA-seq.

Trial Locations

Locations (1)

Wright State Physicians; 🇺🇸 Fairborn, Ohio, United States