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Is Long-Term Proton Pump Inhibitor Use a Cause of Leaky Gut Syndrome

Not Applicable
Completed
Conditions
Proton Pump Inhibitor Adverse Reaction
Interventions
Diagnostic Test: Blood for zonulin level was drawn from the group.
Registration Number
NCT06268834
Lead Sponsor
Dr Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital
Brief Summary

Our study aims to investigate the effect of bacterial overgrowth induced by long-term PPI use on zonulin levels.

Detailed Description

The study included a total of 75 participants, with 45 individuals classified as long-term PPI users and 30 as non-PPI users. The PPI user group was defined as those who had been consistently using PPIs (specifically omeprazole, lansoprazole, pantoprazole, esomeprazole, or rabeprazole) on a daily basis for a minimum of 6 months or at least twice a week for a minimum of 2 years. The control group comprised individuals who had never used PPIs. Venous blood samples were taken from all participants to measure serum zonulin levels.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
75
Inclusion Criteria

The patient group was patients taking long-term PPIs (omeprazole, lansoprazole, pantoprazole, esomeprazole and rabeprazole) every day for at least 6 months or at least twice a week for at least 2 years.

A control group was formed from individuals who had never used PPI.

Exclusion Criteria

Those with inflammatory bowel disease, celiac disease, type 1 diabetes, active gastroenteritis, acute kidney failure, chronic kidney failure, and those who had used antibiotics for any reason in the last 3 months, as well as those receiving probiotic drug support, were not included in the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Using PPIBlood for zonulin level was drawn from the group.Group receiving a long-term PPI (omeprazole, lansoprazole, pantoprazole, esomeprazole and rabeprazole) every day for at least 6 months or at least twice a week for at least 2 years.
ControlBlood for zonulin level was drawn from the group.A control group was formed from individuals who had never used PPI.
Primary Outcome Measures
NameTimeMethod
Zonulin levels in long-term proton pump inhibitor usersBlood was drawn and collected during the examination and studied collectively after 3 months

Approximately 5 cc of blood was drawn from the antebrachial veins of the patients to determine serum zonulin levels. Serum örnekleri -80 santigrat derecede 3 ay süreyle saklanmıştır. Serum örneklerindeki Zonulin seviyeleri Shanghai Crystal Day Biotech Co, Ltd, Shanghai, Çin'den ticari BT-LAB Human Zonulin ELISA kitleri (Kat. No. E1117Hu) kullanılarak ölçülmüştür. The test range of the kit was 2-600 ng/ml. The sensitivity of the test was 1.09 ng/ml. A serum zonulin level above 12 ng/ml was considered positive.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital

🇹🇷

Ankara, Turkey

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