A comparative study of the role of intestinal acetate production in human appetite and insulin secretion and insulin sensitivity
Recruiting
- Conditions
- We will include subjects from the Amsterdam HELIUS study (n=6000) and include only those with 10% lowest and 10% highest fecal butyryl-coenzyme A transferase enzyme (based on previously obtained results of 16S analysis and cross-validated by qPCR butyryl CoA transferase) will be approached for this study.
- Registration Number
- NL-OMON28226
- Lead Sponsor
- AMC-UvA
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 54
Inclusion Criteria
Male or female
- Stable bodyweight (<5% reported weight change during the previous 3 months)
Exclusion Criteria
- Use of corticosteroids, antibiotics and other immunomodulating medication in last 3 months
- XTC, amphetamine, nicotine or cocaine abuse, based on the 20 item drug abuse screening test (DAST-20)
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method - differences in Postprandial plasma acetate levels after a SMMT<br /><br>- differences in Baseline Intestinal microbiota composition (morning stool samples)<br /><br>- differences in baseline Faecal acetate levels
- Secondary Outcome Measures
Name Time Method - CNS (fMRI) responses to food pictures (BOLD) signal, i.e. in CNS activation in predefined regions (insula, striatum, amygdala, OFC, PFC, hippocampus and hypothalamus) in response to viewing food pictures or consuming actual food<br /><br>- Insulin sensitivity measured as Matsuda’s insulin sensitivity index during the standardized mixed meal test<br /><br>- Insuline secretion measured as c-peptide response during the standardized mixed meal test<br /><br>- Energy metabolism, measured by resting energy expenditure (REE)<br /><br>- Baseline physical activity energy expenditure (PAEE)<br /><br>- Dietary intake (https://mijn.voedingscentrum.nl/nl/eetmeter), weight and body<br>composition (Body Impedance Analysis, BIA).<br /><br>- Subjective appetite sensations and eating behavior (VAS questionnaires)<br>