Role of intestinal Acetate Production in human Appetite and Insulin Sensitivity

Completed

Conditions: Obesity and insulin resistance
10018424

Registration Number: NL-OMON46597

Lead Sponsor: Academisch Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 54

Inclusion Criteria

- Part of HELIUS study and has given permission to be approached for new studies
- Stable body weigh (<5% reported weight change during the previous 3 months)
- Age 18-65
- Caucasian
- Able and willing to give informed consent

Exclusion Criteria

- Use of corticosteroids, antibiotics and other immunomodulating medication in last 3 months
- current XTC, amphetamine, nicotine or cocaïne abuse
- Alcohol abuse (>3 units per day)
- Contraindication for MRI (pregnancy, neurological illness, malignancy, history of major heart disease, history of major renal disease, breastfeeding, paecmaker and metals contraindicated for MRI present in the body).
- Expected prolonged compromised immunity (due to recent cytotoxic chemotherapy or HIV infection with a CD4 count <240)
- Presence of inflammatory bowel disease (IBD)
- Inability to understand the study protocol, give informed consent or participate adequately in study protocol.

Study & Design

Study Type: Observational non invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>- Postprandial plasma SCFA including acetate levels after a standardized mixed<br /><br>meal test<br /><br>- Intestinal microbiota composition (morning stool samples)</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- CNS (fMRI) responses to food pictures. The primary outcome is the difference<br /><br>in BOLD signal, i.e. in CNS activation in predefined regions (insula, striatum,<br /><br>amygdala, OFC and hypothalamus) in response to viewing food pictures or<br /><br>consuming actual food<br /><br>- Postprandial plasma glucose and insulin levels<br /><br>- Fasting plasma SCFA, glucose and insulin levels at baseline<br /><br>- Energy metabolism, measured by resting energy expenditure (REE)<br /><br>- Baseline physical activity energy expenditure (PAEE)<br /><br>- Dietary intake (https://mijn.voedingscentrum.nl/nl/eetmeter), weight and body<br /><br>composition (Body Impedance Analysis, BIA).<br /><br>- Subjective appetite sensations and food intake (VAS questionnaires)</p><br>