Oxidative and Antioxidant Indices to HIIT and Aloe Vera Supplementation in Patients With T2DM
- Conditions
- Diabetes Type 2
- Interventions
- Behavioral: ExerciseDietary Supplement: Aloe vera
- Registration Number
- NCT06377657
- Lead Sponsor
- University of Nis
- Brief Summary
Diabetes is a prevalent metabolic disease that leads to increased blood sugar levels due to insulin shortage or resistance. Type 2 diabetes is often linked to obesity, which can increase insulin resistance. Meteorin-like protein (METRNL) is a new secreted protein that affects insulin sensitivity and has been found to be negatively related to serum glucose levels in people with diabetes.
Oxidative stress in diabetics can stimulate the production of inflammatory mediators, increasing the activity of the antioxidant system. Aloe vera, a widely used plant, has been used for treating diabetes, wound healing, tumors, and inflammatory bowel disease. It has anti-inflammatory, antioxidant, neuroprotective, antidepressant, and memory-enhancing effects.
Exercise can improve diabetic patients' conditions by increasing the expression of GLUT4 protein and skeletal muscle. High-intensity interval training has been shown to increase the expression of the METORIN gene in human subjects and decrease MDA in type 2 diabetic patients. However, no studies have examined the simultaneous effect of exercise and Aloe vera on diabetes indicators.
The present study aims to investigate the response of METRNL and some oxidative and antioxidant plasma indicators to high-intensity interval training and Aloe vera supplementation in type 2 diabetic men. The findings indicate that exercise and consumption of plants with anti-inflammatory and antioxidant properties can be effective in modulating the pathological effects of diabetes and increasing insulin sensitivity.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 44
- Male participants who have been diagnosed with T2DM at least 3 months prior to the start of the study according to standard criteria
- Inactive participants according to the International Physical Activity Questionnaire (IPAQ)
- Participants receiving stable medical therapy for T2DM, excluding those on exogenous insulin therapy
- Participants who were free from injuries or chronic health conditions that could limit their ability to safely participate in an intense exercise program were included in the study
- Fasting glucose above 270 mg / dL
- Exogenous insulin
- History of diabetic complications (the final stage of proliferative diabetic retinopathy, or subsequent overt nephropathy, diabetic ketoacidosis, or severe diabetic neuropathy)
- Hereditary glucose and galactose malabsorption
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description High-Intensity Interval Training Exercise Participants in HIIT group underwent 3 sessions per week for 12 weeks. The HIIT protocol (maximum 60 cycles per 20-minute session) includes 8-second fast pedaling (approximately 80% of maximum heart rate (HR max)) followed by slow pedaling (20-30 rpm) for 12 seconds. To facilitate acceleration and limit the stagnation of the bicycle wheel, the selected resistance was very low (almost zero). HIIT + Aloe Vera Exercise Participants in HIIT group underwent 3 sessions per week for 12 weeks. The HIIT protocol (maximum 60 cycles per 20-minute session) includes 8-second fast pedaling (approximately 80% of maximum heart rate (HR max)) followed by slow pedaling (20-30 rpm) for 12 seconds. To facilitate acceleration and limit the stagnation of the bicycle wheel, the selected resistance was very low (almost zero). Additionally, patients underwent supplementation with Aloe Vera capsules in the morning and after the dinner. Aloe Vera supplementation Aloe vera Participants in Aloe Vera group underwent supplementation with Aloe vera capsule containing 100 mg of Aloe vera gel powder in the morning immediately after waking up and one capsule after dinner. HIIT + Aloe Vera Aloe vera Participants in HIIT group underwent 3 sessions per week for 12 weeks. The HIIT protocol (maximum 60 cycles per 20-minute session) includes 8-second fast pedaling (approximately 80% of maximum heart rate (HR max)) followed by slow pedaling (20-30 rpm) for 12 seconds. To facilitate acceleration and limit the stagnation of the bicycle wheel, the selected resistance was very low (almost zero). Additionally, patients underwent supplementation with Aloe Vera capsules in the morning and after the dinner.
- Primary Outcome Measures
Name Time Method METRNL - Meteorin Like, Glial Cell Differentiation Regulator Baseline and 12 weeks Plasma METRNL content was measured using a special kit (Mybiosource company, USA) with a sensitivity of \<0.64 ng / ml, range of 1.56-100 ng / ml by ELISA method
- Secondary Outcome Measures
Name Time Method Total antioxidant blood capacity (TAD) Baseline and 12 weeks TAD was measured using a special kit (Abcam company, USA) through ELISA method.
Glutathione peroxidase (GPx) Baseline and 12 weeks GPx was measured using a special kit (abcam company, USA) through ELISA method.
Serum superoxide dismutase (SOD) Baseline and and 12 weeks SOD was measured using a special kit (Abcam company, USA) through ELISA method.
Plasma malondialdehyde (MDA) Baseline and 12 weeks MDA was measured using a special kit (Abcam company, USA) through ELISA method.
Trial Locations
- Locations (1)
Department of Sport Physiology, Faculty of Sport and Physical education, Islamic Azad University of Ayatollah Amoli, Amol, Iran
🇮🇷Amol, Iran, Islamic Republic of