Plasma Levels of Mitochondrial DNA in Patients Presenting to ICU With Sepsis
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- change from baseline in plasma mtDNA in septic patients at 7 days
研究概览
简要总结
To determine the plasma levels of mtDNA in ICU department patients with and without sepsis and evaluate their association with severity, systemic inflammation and outcomes. Plasma from control, septic and severe septic patients will be collected. The level of mtDNA and systemic cytokine will be measured.
详细描述
Clinical protocol:
Patients were enrolled into one of three cohorts: (i) sepsis defined as suspected infection and systemic inflammatory response, (ii) septic shock defined as sepsis plus hypotension, and (iii) control defined as non-infected patients without systemic inflammatory response.
Methods:
Plasma levels of mtDNAs were measured using real-time quantitative polymerase chain reaction. Levels of mtDNAs were compared among each group, and linear regression was used to assess the association between mtDNAs, IL-6, TNF a and IL-1b in patients with sepsis. The correlation between plasma mtDNA and severity, systemic inflammation and outcomes will be analyzed.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Diagnosis of sepsis and severe sepsis was according to the following guild-line. "Surviving Sepsis Campaign: International Guidelines for Management of Severe Sepsis and Septic Shock: 2012"
排除标准
- •Patients lost following-up. Patients who refuse to sign the consent form.
结局指标
主要结局
change from baseline in plasma mtDNA in septic patients at 7 days
时间窗: 7 days
The change from baseline in plasma mtDNA in septic patients at 7 days will be measured by real-time PCR.
次要结局
- change from baseline in plasma IL-6, TNF a and IL-1b level in septic patients at 7 days(7 days)
研究者
lemeng zhang
md
Central South University