MedPath

Bioavailability of Curcuminoids From Turmipure Gold® in Different Food Matrices

Not Applicable
Completed
Conditions
Healthy
Interventions
Dietary Supplement: TPG capsule
Dietary Supplement: TPG ready to drink
Dietary Supplement: TPG dairy analog
Dietary Supplement: TPG probiotic drink
Dietary Supplement: TPG gummies
Dietary Supplement: TPG sport bar
Registration Number
NCT06300021
Lead Sponsor
Givaudan France Naturals
Brief Summary

The goal of this clinical trial is to learn about the extent to which turmeric compounds in different food matrices becomes available to the body in healthy volunteers. The main question it aims to answer is:

* Is the availability to the body of a turmeric dietary supplement altered when consumed in fruit nectar, oat milk, yogurt drink, or gummies in comparison to a dietary supplement capsule?

Participants will ingest a turmeric dietary supplement as a capsule or in different food matrices on six testing days (separated by at least one week). The day before testing day, a standardized dinner will be provided to the participants in the evening and participants will be instructed to not consume any foods or beverages (except water) and to arrive fasted (12 hours) at the investigation site. Participants will ingest turmeric dietary supplement formulations one by one in random order in the morning of testing days. Regular blood and urine samples will be collected before and up to 24 hours after the turmeric dietary supplement ingestion. Standardized meals will be provided during the entire testing day (lunch, mid-afternoon snack, dinner).

Detailed Description

Curcumin has been studied as the main bioactive component of the rhizome of the herb Curcuma longa, known as turmeric, and is associated to potential health effects and benefits. Other bioactive components have also been identified, called the curcuminoid group. Curcuminoids are natural yellow-orange pigments and hydrophobic polyphenols derived from. Extracts of turmeric generally contain 75-80% curcumin, 15-20% demethoxycurcumin (DMC), and 0-10% bisdemethoxycurcumin (BDMC). Curcumin and curcuminoids have been extensively investigated due to their antioxidant and anti-inflammatory properties, notably regarding their potential efficacy in modulating various health conditions.

In the present trial, the aim is to assess the bioavailability of curcuminoids in different food matrix formulations, the capsule format being the reference matrix, with a hypothesis of equivalence. The primary objective of the study is to assess the plasmatic concentration profile of total curcuminoids (curcumin, DMC, BDMC and their metabolites) on a 24-hour period after consumption of five different food matrix formulations containing TPG in comparison to the dietary supplement capsule formulation.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
35
Inclusion Criteria
  • Age between 18 and 45 years (limits included)
  • BMI between 18.5 and 24.9 kg/m² (limits included)
  • Weight stable within ±3 kg in the last three months
  • With routine blood chemistry values within the normal range
  • For women: Non menopausal with the same reliable contraception since at least 3 cycles before the beginning of the study and agreeing to keep it during the entire duration of the study or menopausal without or with hormone replacement therapy since at least 3 months
  • Non-smoking or with tobacco consumption ≤5 cigarettes per day and agreeing not to smoke during the entire duration of the study
  • Good general and mental health in the opinion of the investigator: no clinically significant and relevant abnormalities of medical history or physical examination
  • Able and willing to participate to the study by complying with the protocol procedures as evidenced by dated and signed informed consent form
  • Affiliated with a social security scheme
  • Agreeing to be registered on the volunteers in biomedical research file
Exclusion Criteria
  • Suffering from a metabolic or endocrine disorder such as diabetes, uncontrolled or controlled thyroidal trouble or other metabolic disorder
  • Suffering from a chronic disease (e.g. cancer, HIV, kidney failure, ongoing hepatic or biliary disorders, chronic inflammatory digestive disease, arthritis, anemia or other chronic respiratory trouble, gammapathies, hepatic diseases, decompensated diabetes, tuberculosis, ulcerative colitis, parkinson's disease, etc.) or gastrointestinal disorders found to be inconsistent with the conduct of the study by the investigator (e.g. celiac disease)
  • Suffering from liver diseases
  • Having medical history of current pathology which could affect the study results or expose the subject to an additional risk according to the investigator (IBS, chronic diarrhea, constipation or abdominal pain, inflammatory bowel diseases (Crohn's disease or ulcerative colitis), cirrhosis, chronic laxatives use, severe COVID-19 history (requiring oxygen-therapy) in the 12 past months, ...)
  • Recent gastroenteritis or food borne illness such as confirmed food poisoning (less than 1 month)
  • With a low venous capital of blood samples according to the investigator's opinion
  • With a known or suspected allergy or intolerance to any food
  • With a known or suspected food allergy or intolerance or hypersensitivity to any of the study products' ingredient or turmeric and/or of the standard meals
  • Pregnant or lactating women or intending to become pregnant within 3 months ahead
  • Currently taking any chronic drug treatment (for example: antidiabetics, anticoagulant, antihypertensive treatment, treatment thyroid, asthma treatment, anxiolytic, antidepressant, lipid-lowering treatment, corticosteroids, phlebotonic, veinotonic, drug with impact on blood circulation, ...) except oral and local contraceptives,
  • Currently taking or having taken any supplementation from botanical origins or with curcumin in the previous 3 months before inclusion
  • Currently taking or having taken laxatives or antibiotics, in the previous 3 months before the V0 visit
  • Currently taking or having taken any prebiotics or probiotics supplementation from food or from dietary supplements in the previous 3 months before the V0 visit
  • With significant change in food habits or in physical activity in the 3 months before the V0 visit or not agreeing to keep them unchanged throughout the study
  • Trying to lose weight with a current or planned in the next 3 months specific diet (hyper- or hypocaloric, vegan, vegetarian, ...) or exercise regimen
  • With a personal history of anorexia nervosa, bulimia or significant eating disorders according to the investigator
  • Exhibiting alcohol or consuming more than 3 standard drinks of alcoholic beverage daily for men or 2 daily for women or not agreeing to keep alcohol consumption habits unchanged throughout the study or drug dependence or exhibiting drug dependence
  • Having a lifestyle deemed incompatible with the study according to the investigator including high level physical activity (defined as more than 5 hours of significant physical activity a week, walking excluded)
  • Having taken part in another clinical trial in the previous 3 months or being in the exclusion period of a previous clinical trial
  • Having received, during the last 12 months, indemnities for clinical trial higher or equal to 4500 Euros
  • Under legal protection (guardianship, wardship) or deprived from rights following administrative or judicial decision
  • Presenting a psychological or linguistic incapability to sign the informed consent
  • Impossible to contact in case of emergency
  • Having consumed curcumin-containing food supplements (curcumin, turmeric and curry) or foods (curcumin, turmeric, E100, and curry) defined as at least 3 times per week and for 2 weeks prior to testing
  • Made a blood donation in the 3 months before the V0 visit or intending to make it within 3 months ahead
  • After V0 biological analyses the subject will be considered as non-eligible to the study on the following criteria: Control record (glycaemia, total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol, GGT, ASAT, ALAT, urea, creatinine, total bilirubin and CBC) with clinically significant abnormality according to the investigator

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Sequence ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummiesTPG dairy analogSubjects receive Turmipure Gold® preparations in different food matrices in the following order: ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummies
Sequence capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drinkTPG capsuleSubjects receive Turmipure Gold® preparations in different food matrices in the following order: capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drink
Sequence capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drinkTPG dairy analogSubjects receive Turmipure Gold® preparations in different food matrices in the following order: capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drink
Sequence capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drinkTPG sport barSubjects receive Turmipure Gold® preparations in different food matrices in the following order: capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drink
Sequence dairy analog - probiotic drink - ready to drink - gummies - capsule - sport barTPG capsuleSubjects receive Turmipure Gold® preparations in different food matrices in the following order: dairy analog - probiotic drink - ready to drink - gummies - capsule - sport bar
Sequence gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drinkTPG sport barSubjects receive Turmipure Gold® preparations in different food matrices in the following order: gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drink
Sequence ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummiesTPG capsuleSubjects receive Turmipure Gold® preparations in different food matrices in the following order: ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummies
Sequence capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drinkTPG ready to drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drink
Sequence capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drinkTPG gummiesSubjects receive Turmipure Gold® preparations in different food matrices in the following order: capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drink
Sequence dairy analog - probiotic drink - ready to drink - gummies - capsule - sport barTPG ready to drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: dairy analog - probiotic drink - ready to drink - gummies - capsule - sport bar
Sequence dairy analog - probiotic drink - ready to drink - gummies - capsule - sport barTPG dairy analogSubjects receive Turmipure Gold® preparations in different food matrices in the following order: dairy analog - probiotic drink - ready to drink - gummies - capsule - sport bar
Sequence dairy analog - probiotic drink - ready to drink - gummies - capsule - sport barTPG probiotic drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: dairy analog - probiotic drink - ready to drink - gummies - capsule - sport bar
Sequence dairy analog - probiotic drink - ready to drink - gummies - capsule - sport barTPG gummiesSubjects receive Turmipure Gold® preparations in different food matrices in the following order: dairy analog - probiotic drink - ready to drink - gummies - capsule - sport bar
Sequence ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummiesTPG sport barSubjects receive Turmipure Gold® preparations in different food matrices in the following order: ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummies
Sequence capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drinkTPG probiotic drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: capsule - ready to drink - sport bar - dairy analog - gummies - probiotic drink
Sequence gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drinkTPG capsuleSubjects receive Turmipure Gold® preparations in different food matrices in the following order: gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drink
Sequence probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsuleTPG gummiesSubjects receive Turmipure Gold® preparations in different food matrices in the following order: probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsule
Sequence ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummiesTPG ready to drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummies
Sequence sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analogTPG ready to drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analog
Sequence sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analogTPG probiotic drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analog
Sequence sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analogTPG sport barSubjects receive Turmipure Gold® preparations in different food matrices in the following order: sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analog
Sequence dairy analog - probiotic drink - ready to drink - gummies - capsule - sport barTPG sport barSubjects receive Turmipure Gold® preparations in different food matrices in the following order: dairy analog - probiotic drink - ready to drink - gummies - capsule - sport bar
Sequence gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drinkTPG dairy analogSubjects receive Turmipure Gold® preparations in different food matrices in the following order: gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drink
Sequence gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drinkTPG probiotic drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drink
Sequence gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drinkTPG gummiesSubjects receive Turmipure Gold® preparations in different food matrices in the following order: gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drink
Sequence probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsuleTPG capsuleSubjects receive Turmipure Gold® preparations in different food matrices in the following order: probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsule
Sequence probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsuleTPG dairy analogSubjects receive Turmipure Gold® preparations in different food matrices in the following order: probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsule
Sequence probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsuleTPG sport barSubjects receive Turmipure Gold® preparations in different food matrices in the following order: probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsule
Sequence ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummiesTPG probiotic drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummies
Sequence ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummiesTPG gummiesSubjects receive Turmipure Gold® preparations in different food matrices in the following order: ready to drink - dairy analog - capsule - probiotic drink - sport bar - gummies
Sequence sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analogTPG capsuleSubjects receive Turmipure Gold® preparations in different food matrices in the following order: sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analog
Sequence gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drinkTPG ready to drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: gummies - sport bar - probiotic drink - capsule - dairy analog - ready to drink
Sequence probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsuleTPG ready to drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsule
Sequence probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsuleTPG probiotic drinkSubjects receive Turmipure Gold® preparations in different food matrices in the following order: probiotic drink - gummies - dairy analog - sport bar - ready to drink - capsule
Sequence sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analogTPG gummiesSubjects receive Turmipure Gold® preparations in different food matrices in the following order: sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analog
Sequence sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analogTPG dairy analogSubjects receive Turmipure Gold® preparations in different food matrices in the following order: sport bar - capsule - gummies - ready to drink - probiotic drink - dairy analog
Primary Outcome Measures
NameTimeMethod
Dose-normalized AUC0-24h of total curcuminoids0 to 24 hours

Normalized (according to mg of curcuminoids ingested) area under the concentration-time curve from 0 to 24 h of total curcuminoids (sum of all quantified metabolites) in plasma:

* Total curcuminoids = curcumin + demethoxycurcumin (DMC) + bisdemethoxycurcumin (BDMC) + tetrahydrocurcumin (THC) + hexahydrocurcumin (HHC) + curcumin glucuronide + DMC glucuronide + BDMC glucuronide + THC glucuronide + HHC glucuronide + curcumin sulfate + DMC sulfate + BDMC sulfate + THC sulfate + HHC sulfate

Secondary Outcome Measures
NameTimeMethod
AUC0-∞ of total curcuminoids, curcuminoid compounds and of their metabolitesData collected from 0 to 24 hours

Area under the plasma concentration-time curve from 0 hours to extrapolated infinite time of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Half-life of total curcuminoids, curcuminoid compounds and of their metabolites0 to 24 hours

Time after which the plasma concentration decreased by half of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Tmax of total curcuminoids, curcuminoid compounds and of their metabolites0 to 24 hours

Time to peak plasma concentration of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

AUC0-8h of total curcuminoids, curcuminoid compounds and of their metabolites0 to 8 hours

Area under the plasma concentration-time curve from 0 to 8 h of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Relative bioavailability for normalized AUC0-8h of total curcuminoids, curcuminoid compounds and of their metabolites0 to 8 hours

Relative bioavailability (ratio of the dose-normalized AUCs) in different food matrices versus capsule format of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Cmax of total curcuminoids, curcuminoid compounds and of their metabolites0 to 24 hours

Peak plasma concentration of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Dose-normalized AUC0-24h of curcuminoid compounds and of their metabolites0 to 24 hours

Normalized (according to mg of curcuminoids ingested) area under the plasma concentration-time curve from 0 to 24 h of:

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Dose-normalized AUC0-8h of total curcuminoids, curcuminoid compounds and of their metabolites0 to 8 hours

Normalized (according to mg of curcuminoids ingested) area under the plasma concentration-time curve from 0 to 8 h of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Dose-normalized AUC0-∞ of total curcuminoids, curcuminoid compounds and of their metabolitesData collected from 0 to 24 hours

Normalized (according to mg of curcuminoids ingested) area under the plasma concentration-time curve from 0 hours to extrapolated infinite time of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Dose-normalized Cmax of total curcuminoids, curcuminoid compounds and of their metabolites0 to 24 hours

Normalized (according curcuminoids ingested) peak plasma concentration of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

AUC0-24h of total curcuminoids, curcuminoid compounds and of their metabolites0 to 24 hours

Area under the plasma concentration-time curve from 0 to 24 h of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Relative bioavailability for normalized AUC0-24h of total curcuminoids, curcuminoid compounds and of their metabolites0 to 24 hours

Relative bioavailability (ratio of the dose-normalized AUCs) in different food matrices versus capsule format of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Relative bioavailability for normalized AUC0-∞ of total curcuminoids, curcuminoid compounds and of their metabolitesData collected from 0 to 24 hours

Relative bioavailability (ratio of the dose-normalized AUCs) in different food matrices versus capsule format of:

* Total curcuminoids

* Total parent compounds

* Total sulfate metabolites

* Total glucuronide metabolites

* Total parent compounds and their relative sulfate and glucuronide metabolites

* Curcumin and its relative sulfate and glucuronide metabolites

* DMC and its relative sulfate and glucuronide metabolites

* BDMC and its relative sulfate and glucuronide metabolites

* Curcumin and all its relative metabolites

Trial Locations

Locations (1)

Biofortis

🇫🇷

Saint-Herblain, France

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