Pilot study: A comparison of the extent of haemolysis induced by conventional versus miniaturised extracorporeal circulation during coronary artery bypass grafting surgery, and the influence on the release of damage associated molecular patterns (DAMPs), leukocyte activation and neutrophil extracellular trap (NET) formation.<br>
Completed
- Conditions
- cell dead of red blood cellshaemolysis100189111001108210007593
- Registration Number
- NL-OMON45709
- Lead Sponsor
- Amphia Ziekenhuis
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 40
Inclusion Criteria
Elective CABG operation with extracorporeal circulation
Exclusion Criteria
o Kidney insufficiency: creatinine >150 µmol/L
o Liver insufficiency: ASAT >80 U/L
o Left ventricular function (LVF): <30% Ejection fraction (EF)
o Body surface area (BSA): <1.6; *2.1 m²
o Carotid artery stenosis
o Pre-operative Haemoglobin: <7,5; *10 mmol/L
o Pre-operative red blood cell transfusion: <14 days before CABG procedure
o Splenectomy (influences the IgM levels used to determine the amount of haemodilution)
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main study parameter is the amount of haemolysis as examined by determining<br /><br>cell free haem/cell free haemoglobin, haemoglobin, haematocrit and lactate<br /><br>dehydrogenase. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary study parameters are markers for the release of DAMPs (nucleosomes,<br /><br>cell free DNA HMGB1), the activation of leukocytes (calprotectin) and<br /><br>neutrophils (human neutrophil elastase-alpha1 antitrypsin complexes) and the<br /><br>formation of neutrophil extracellular traps (myeloperoxidase(MPO)-nucleosome<br /><br>complexes). </p><br>