Acute Effect of Fructose on Lipid Metabolism and Gender Differences
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 18
- 试验地点
- 2
- 主要终点
- Hepatic de novo lipogenesis
研究概览
简要总结
It has been widely documented that fructose overfeeding increases plasma triglycerides and hepatic de novo lipogenesis, and impairs insulin sensitivity in healthy male volunteers. The effect of gender on the metabolic responses to fructose remains an important open question, however.
The objective of this study is to compare the effect of an acute oral fructose load on carbohydrate and lipid metabolism in healthy young males and females.
详细描述
The study is aimed at comparing the effects of oral fructose on several specific metabolic pathways in males and females.Participants will receive an isoenergetic diet containing 55% carbohydrate, 15% protein and 35% lipids for three days prior to testing. After this period of controlled diet, they will be studied for 2 hours in the post-absorptive state (Time 0-120 min) and over a 6 hours period (Time 120-480 min) during which they will receive 4 loads of 0,30 g/kg fat free mass U-13C labelled fructose, at times 120, 180, 240, 300. Throughout the study, deuterated glucose and glycerol will be infused to monitor whole body glucose production and glycerol turnover.
The following parameters will be monitored in basal conditions and after the ingestion of the load of fructose:
- Glycerol turnover(glycerol 2H5)
- de novo lipogenesis (incorporation of 13C into palmitate of VLDLs)
- whole body energy expenditure and net substrate oxydation (indirect calorimetry)
- net fructose oxidation (breath 13CO2)
- glucose turnover: (6,6 2H2 Glucose)
- plasma glucose, free fatty acids, ketone bodies, lactate, insulin, triacylglycerol, total cholesterol, VLDL, LDL, and HDL subfractions
An adipose tissue (periumbilical subcutaneous) biopsy will be obtaine by needle aspiration under local anesthesia in fasting conditions (time 0 min) and after fructose (time 480 min) to assess the effects of fructose on adipose gene expression profile. Key genes involved in the regulation of carbohydrate (GLUT 4, hexokinase, PDH-kinase), lipid (FAT-CD36, FABP, acetylCoA carboxylase, malonyl-CoA decarboxylase, PPARg) and energy metabolism (PGC-1a, UCP2)will be monitored
Results obtained in males and females will be compared with two-way analysis of variance
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and females
- •Age 18-35
- •Body mass indexes (BMI) between 19 and 25 kg/m2
- •Informed consent obtained
排除标准
- •Alcohol intake > 30g/day
- •Drug abuse
- •Diabetes mellitus
研究组 & 干预措施
fructose
acute fructose administration
干预措施: fructose (Dietary Supplement)
结局指标
主要结局
Hepatic de novo lipogenesis
时间窗: acute effect of dietary fructose (within 6 hours)
次要结局
- Whole body lipid oxidation, glucose turnover, glycerol turnover, plasma substrates, hormone and energy expenditure (free fatty acid, glucose, lactate, beta-hydroxybutyrate, glycerol, VLDL- Triglycerides and insulin) expression of key adipose genes(acute effect of fructose (within 6 hours))
研究者
Luc Tappy, MD
Professor of physiology
University of Lausanne