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A Study of PEGASYS (Peginterferon Alfa-2a (40KD)) Plus COPEGUS (Ribavirin) With or Without Pioglitazone in Treatment-Naive Patients With Chronic Hepatitis C and Insulin Resistance.

Phase 4
Completed
Conditions
Hepatitis C, Chronic
Interventions
Drug: peginterferon alfa-2a [Pegasys]
Registration Number
NCT00545233
Lead Sponsor
Hoffmann-La Roche
Brief Summary

This 2 arm study will assess the efficacy and safety of PEGASYS plus COPEGUS, with or without concomitant pioglitazone, on hepatitis C virus titers in treatment-naive patients with genotype 1 chronic hepatitis C, and insulin resistance. Patients will be randomized to receive either a)PEGASYS 180 micrograms/week + Copegus 1000-1600 mg/day (according to body weight) for 48 weeks or b)16 weeks of pioglitazone (30 mg daily for 8 weeks, then 45 mg daily for 8 weeks), followed by PEGASYS 180 micrograms/week + Copegus 1000-1600 mg/day + pioglitazone 45 mg daily for 48 weeks. The anticipated time on study treatment is 1-2 years, and the target sample size is 100-500 individuals.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
155
Inclusion Criteria
  • adult patients, >=18 years of age;
  • chronic hepatitis C, genotype 1;
  • insulin resistance.
Exclusion Criteria
  • other forms of liver disease;
  • cirrhosis;
  • previous treatment for chronic hepatitis C;
  • insulin treatment during prior 2 weeks;
  • type 1 diabetes.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PEG-INF alpha-2a + ribavirin+ pioglitazonepeginterferon alfa-2a [Pegasys]Participants received pioglitazone in a 16 week run-in period (30 mg per day orally for 8 weeks followed by 45 mg per day orally for 8 weeks). Participants then received piogliatzone 45 mg daily orally plus 18 μg of Peginterferon Alfa-2a (PEG-INF alpha-2a) subcutaneous (sc) once a week plus ribavirin (1000 - 1600 mg/day orally as a split dose in the morning and the evening based on the participant's body weight) for 48 weeks in the anti-HCV treatment phase. Participants received 45 mg of pioglitazone per day orally in the 24 week follow-up period.
PEG-INF alpha-2a + ribavirin+ pioglitazoneribavirin [Copegus]Participants received pioglitazone in a 16 week run-in period (30 mg per day orally for 8 weeks followed by 45 mg per day orally for 8 weeks). Participants then received piogliatzone 45 mg daily orally plus 18 μg of Peginterferon Alfa-2a (PEG-INF alpha-2a) subcutaneous (sc) once a week plus ribavirin (1000 - 1600 mg/day orally as a split dose in the morning and the evening based on the participant's body weight) for 48 weeks in the anti-HCV treatment phase. Participants received 45 mg of pioglitazone per day orally in the 24 week follow-up period.
PEG-INF alpha-2a + ribavirinpeginterferon alfa-2a [Pegasys]Participants received 18 μg of Peginterferon Alfa-2a (PEG-INF alpha-2a) subcutaneous (sc) once a week plus ribavirin (1000 - 1600 mg/day orally as a split dose in the morning and the evening based on the participant's body weight) for 48 weeks in the anti-HCV treatment phase followed by a treatment free 24 week follow-up period.
PEG-INF alpha-2a + ribavirinribavirin [Copegus]Participants received 18 μg of Peginterferon Alfa-2a (PEG-INF alpha-2a) subcutaneous (sc) once a week plus ribavirin (1000 - 1600 mg/day orally as a split dose in the morning and the evening based on the participant's body weight) for 48 weeks in the anti-HCV treatment phase followed by a treatment free 24 week follow-up period.
PEG-INF alpha-2a + ribavirin+ pioglitazonePioglitazoneParticipants received pioglitazone in a 16 week run-in period (30 mg per day orally for 8 weeks followed by 45 mg per day orally for 8 weeks). Participants then received piogliatzone 45 mg daily orally plus 18 μg of Peginterferon Alfa-2a (PEG-INF alpha-2a) subcutaneous (sc) once a week plus ribavirin (1000 - 1600 mg/day orally as a split dose in the morning and the evening based on the participant's body weight) for 48 weeks in the anti-HCV treatment phase. Participants received 45 mg of pioglitazone per day orally in the 24 week follow-up period.
Primary Outcome Measures
NameTimeMethod
Change From Initiation of Pegasys Plus Copegus in log10 Hepatitis C Virus Ribonucleic Acid (HCV RNA) Viral Load to Week 12 of Anti-HCV TherapyInitiation of Pegasys plus Copegus, Week 12 of anti-HCV treatment

Serum samples were collected for HCV RNA. The change from initiation of Pegasys plus Copegus to Week 12 in HCV RNA titers were calculated. Randomization for the with Pioglitazone arm occurred prior to the 16 week run-in period and randomization for the without Pioglitazone arm occurred prior to the start of anti-HCV treatment.

Secondary Outcome Measures
NameTimeMethod
Change From Initiation of Pegasys Plus Copegus in log10 HCV RNA Viral Load to Week 24 and Week 48 of Anti-HCV TherapyInitiation of Pegasys Plus Copegus, Week 24 and Week 48 of anti-HCV therapy

Serum samples were collected for HCV RNA. The change from Initiation of Pegasys Plus Copegus to Week 24 and Week 48 in HCV RNA titers were calculated. Randomization for the with Pioglitazone arm occurred prior to the 16 week run-in period and randomization for the without Pioglitazone arm occurred prior to the start of anti-HCV treatment.

Percentage of Participants Achieving Virologic ResponseWeeks 4, 12, 24, 48, 60, 72

Virologic response was defined as undetectable HCV RNA \< 28 IU/mL. Patients with missing HCV RNA values are considered as non-responders.

Percentage of Participants With a ≥ 2 log10 Decrease in HCV RNA From Initiation of Pegasys Plus Copegus to Weeks 4, 12, 24, 48, 60, 72Initiation of Pegasys plus Copegus, Weeks 4, 12, 24, 48, 60, 72

Serum samples were collected for HCV RNA. The percentage of participants with a ≥ 2 log10 decrease in HCV RNA from initiation of Pegasys plus Copegus to time point was calculated.

Percentage of Participants With a Virological Relapse at Week 72 (24 Weeks After the End of Anti-HCV Treatment)Week 72

Virologic relapse was defined as the reappearance of HCV-RNA in serum after PEG-INF alpha 2a therapy is discontinued in a patient who was HCV-RNA undetectable at the completion of anti-HCV therapy.

Percentage of Participants With a Confirmed Virological Breakthrough up to 48 WeeksUp to 48 Weeks

Virological breakthrough is a detectable HCV RNA at any time during anti-HCV treatment up to Week 48 after the attainment of undetectable HCV RNA.

Percentage of Nonresponders During the 48 Week Anti-HCV Treatment PeriodUp to 48 Weeks

Nonresponders are defined as patients who did not achieve undetectable HCV RNA during anti-HCV treatment

Change in Log10 HCV RNA Viral Load at Assessments From Randomization to 16 Weeks of Pioglitazone Pretreatment Run-In Period for the Pioglitazone Arm OnlyRandomization (Week-16),Weeks -12, -8, -4 and 0

Serum HCV RNA was collected at randomization and during the pioglitazone run-in period at various time points for the with pioglitazone arm only. The change from randomization to each of these time points was calculated.

Change From Baseline in Fasting Plasma Glucose Levels at Each Time Point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for plasma fasting glucose levels at various time points throughout the study and was used as a measure of glycemic control (monitoring the ability of the patient to maintain normal blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Fasting Insulin Levels at Each Time Point Assessed.Baseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for fasting insulin levels at various time points throughout the study and was used as a measure of glycemic control (monitoring the ability of the patient to maintain normal blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Fasting Hemoglobin A1C (HbA1c) Concentrations at Each Time Point AssessedBaseline, Weeks 4,8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for a fasting Hemoglobin A1C level at various time points throughout the study and was used as a measure of glycemic control (monitoring the ability of the patient to maintain normal blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Homeostasis Model Assessment (HOMA) Scores at Each Time Point AssessedBaseline, Weeks 4,8,12,16,20,24,28,32,36,40,44,48,60,72

Insulin resistance (IR) is calculated using the following formula:

HOMA score = (fasting glucose in mg/dL × fasting insulin in μIU/mL) / 405.

Baseline for "with pioglitazone" arm occurred prior to the start of 16 week run-in period and for "without pioglitazone" arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the start of anti-HCV therapy is calculated.

A normal patient can have a HOMA score up to 3. A patient with a score of \>3 is definitely IR. Patients scoring 2-3 can be IR but other factors may be causing this without being IR.

Change From Baseline in Serum Triglyceride Concentrations at Each Time-point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60, 72

Blood was collected and assayed for fasting serum triglyceride levels at various time points throughout the study and was used as an indicator of lipid control.

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Total Cholesterol Levels at Each Time-point AssessedBaseline, Weeks 4,8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected and assayed for fasting serum cholesterol levels at various time points throughout the study and was used as an indicator of lipid control.

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Low-density Lipoprotein (LDL-cholesterol) Levels at Each Time-point AssessedBaseline, Weeks 4,8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected and assayed for fasting serum low-density lipoprotein (LDL-cholesterol) levels at various time points throughout the study and was used as an indicator of lipid control.

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in High-density Lipoprotein (HDL-cholesterol) Levels at Each Time-point AssessedBaseline, Weeks 4,8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected and assayed for fasting high-density lipoprotein (HDL-cholesterol) levels at various time points throughout the study and was used as an indicator of lipid control.

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Tumor Necrosis Factor Alpha (TNF-α) at Each Time Point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for tumor necrosis factor alpha at various time points throughout the study and was used as a measure of insulin resistance (the inability of insulin to control blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Transforming Growth Factor Beta (TGF-β) Levels at Each Time Point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for Transforming Growth Factor beta at various time points throughout the study and was used as a measure of insulin resistance (the inability of insulin to control blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Adiponectin Levels at Each Time Point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for adiponectin at various time points throughout the study and was used as a measure of insulin resistance (the inability of insulin to control blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Leptin Levels at Each Time Point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for leptin at various time points throughout the study and was used as a measure of insulin resistance (the inability of insulin to control blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Change From Baseline in Free Fatty Acid Levels at Each Time Point AssessedBaseline, Weeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

Blood was collected for free fatty acids at various time points throughout the study and was used as a measure of insulin resistance (the inability of insulin to control blood sugar levels).

Baseline for the with pioglitazone arm occurred prior to the start of 16 week run-in period and for the without pioglitazone arm prior to the start of anti-HCV therapy. The change from baseline to Weeks 4 thru 72 after the initiation of anti-HCV therapy is calculated.

Percentage of Participants With Beck Depression Inventory Fast Screen (BDI-FS) Score ≥ 4 at Each Time Point AssessedWeeks 4, 8,12,16,20,24,28,32,36,40,44,48,60,72

The BDI-FS consisted of seven areas with four statements (labeled 0, 1, 2, and 3) offered to describe the area of interest, with 0 indicating no effect and 3 indicating the worst effect. The individual area scores were summed to provide a total score. The degree of depression was assessed with 0 to 3 indicating minimal depression, 4 to 8 mild depression, 9 to 12 moderate depression and 13 to 21 severe depression.

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